A first catalog of genes involved in pig ovarian follicular differentiation

• Published in: Mammalian genome Vol. 8; no. 4; pp. 250 - 254
• Main Authors: Tosser-Klopp, G, Benne, F, Bonnet, A, Mulsant, P, Gasser, F, Hatey, F
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.04.1997; Springer Verlag
• Subjects: Animals; Cell Differentiation - genetics; Cells, Cultured; Cloning, Molecular; Databases, Factual; DNA probes; DNA, Complementary; Expressed sequence tags; Female; Follicle Stimulating Hormone - physiology; Follicle-stimulating hormone; Gene Expression Regulation; Gene Library; Genetics; Genomics; Granulosa Cells - cytology; Humans; Hybridization; Life Sciences; Molecular Sequence Data; Nucleotide sequence; RNA probes; RNA, Messenger - genetics; Swine; BANQUE DE GENES; FSH
• ISSN: 0938-8990; 1432-1777
• DOI: 10.1007/s003359900403

As a first step toward the characterization of genetic expression in pig ovaries, we have selected 238 clones by differential hybridization from a pig granulosa cell cDNA library, using probes prepared from RNA extracted from either untreated or FSH-treated cells and, in order to generate expressed sequence tags (ESTs), we have performed 3' and 5' single-pass sequencing of these clones. Sequences of the 3' end of the 167 clones that produced informative sequence data were first compared with each other, revealing a redundancy level of 21%. Sequences from the 136 unique clones were analyzed for similarities with sequence data included in Genbank and EMBL databases. Among these unique clones, 54 (40%) matched significantly with sequences from either Genbank of EMBL: 4 with known genes in pig, 35 matched with previously reported human genes, and 15 with other mammalian genes. Eighty-two clones (60%) showed no significant match with any gene or DNA sequence in the Genbank and EMBL databases and thus may represent new pig transcripts.