H2O2 Is Involved in the Metallothionein-Mediated Rice Tolerance to Copper and Cadmium Toxicity

• Published in: International journal of molecular sciences Vol. 18; no. 10; p. 2083
• Main Authors: Zhang, Hongxiao, Lv, Shufang, Xu, Huawei, Hou, Dianyun, Li, Youjun, Wang, Fayuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2017; MDPI
• Subjects: Antioxidants; Cadmium; Cadmium - metabolism; Cadmium - toxicity; Copper; Copper - metabolism; Copper - toxicity; Gene Expression Regulation, Plant; Hydrogen Peroxide - metabolism; Metallothionein - genetics; Metallothionein - metabolism; Oryza - genetics; Oryza - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Reactive oxygen species; Rice; Toxicity; Cd stress; rice suspension cells; ricMT; H2O2; Cu stress
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18102083

Cadmium (Cd) and excess copper (Cu) are toxic to plants, causing a wide range of deleterious effects including the formation of reactive oxygen species. Metallothioneins (MTs) may protect plant cells from heavy metal toxicity by chelating heavy metals via cysteine thiol groups. They may also function as antioxidants. The study investigated the relationship of H2O2 production and ricMT expression in rice radicles and rice suspension cells under Cu or Cd stress. The results showed that H2O2 production in the rice radicles increased before Cu-induced ricMT expression, and after Cd-induced ricMT expression. Rice suspension cells of sense- and antisense-ricMT transgenic lines were obtained by an Agrobacterium-mediated transformation. Overexpression of ricMT significantly decreased the death rate of rice cells, which was accompanied by blocked H2O2 accumulation in rice suspension cells subject to Cu and Cd stress. Our findings confirm that H2O2 is involved in the MT-mediated tolerance of Cu and Cd toxicity in rice.