High-performance liquid chromatography determination of phenolic components in wine using off-line isotachophoretic pretreatment

• Published in: Journal of Chromatography A Vol. 1040; no. 2; pp. 179 - 184
• Main Authors: Sladkovský, Radek, Solich, Petr, Urbánek, Marek
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.06.2004; Elsevier
• Subjects: Analysis; Analytical biochemistry: general aspects, technics, instrumentation; Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid - methods; Electrophoresis - methods; Exact sciences and technology; Fermented food industries; Food analysis; Food industries; Fundamental and applied biological sciences. Psychology; General pharmacology; Isotachophoresis; Medical sciences; Organic acids; Pharmacology. Drug treatments; Phenolic compounds; Phenols - analysis; Sample preparation; Sensitivity and Specificity; Wine; Wine - analysis; Wines and vinegars; Wine; Sample preparation; Isotachophoresis; Food analysis; Phenolic compounds; Organic acids; Analysis; HPLC chromatography; Phenols; Determination; Pretreatment; Food
• ISSN: 0021-9673
• DOI: 10.1016/j.chroma.2004.04.009

The off-line combination of isotachophoresis (ITP) and high-performance liquid chromatography (HPLC) to improve sample pretreatment and determination of phenolic compounds in wine was investigated. The ITP system provided an enhanced sample load capacity and served as a sample clean-up technique, HPLC performed a final separation of the analytes presented in samples. The phenolic components were separated by Discovery RP Amid C 16 chromatography column using water–methanol–acetonitrile–orthophosphoric acid gradient. The identification of phenolic compounds was made by comparison of the retention data obtained for the standard mixture, pretreated sample and the sample spiked standard additions. Satisfactory recoveries for all components analysed were observed between 86.1 and 109.2%.