Utility of expression of 4-hydroxynonenal tested by immunohistochemistry for cervical cancer

• Published in: Przegla̜d menopauzalny Vol. 23; no. 1; pp. 6 - 13
• Main Authors: Tsoneva, Eva, Dimitrova, Polina, Metodiev, Metodi, Shivarov, Velizar, Vasileva-Slaveva, Mariela, Yordanov, Angel, Kostov, Stoyan
• Format: Journal Article
• Language: English
• Published: Poland Termedia Publishing House 01.03.2024
• Subjects: 4-hne; cervical cancer; lipid metabolism; Original Paper; oxidative stress; ros; 4-HNE; cervical cancer; oxidative stress; ROS; lipid metabolism
• ISSN: 1643-8876; 2299-0038
• DOI: 10.5114/pm.2024.136356

Cervical cancer (CC) is a leading cause of mortality in women around the world, with the highest incidence rate still being in developing countries. The most common aetiological factor is infection with high-risk human papilloma virus viral strains. Oxidative stress through generation of reactive oxygen species leads to lipid peroxidation and DNA damage. Studies show that reactive lipid electrophiles such as 4-hydroxynonenal (4-HNE) produced in the process play an important role in cancer signalling pathways and are a good biomarker for oxidative stress. We aim to investigate the prognostic role of 4-HNE as a biomarker for oxidative stress in patients in early and advanced stages of CC measured by immunohistochemistry. This is a retrospective study of 69 patients treated at our Department of Oncogynaecology. Paraffin embedded tumour tissues were immunohistochemically tested for the levels of expression of 4-HNE. The results for H-score, Allred score, and combined score were investigated for association with tumour size, lymph node status, andInternational Federation of Gynaecology and Obstetrics stage. 4-hydroxynonenal showed higher expression in more advanced stages of CC and in cases with involved lymph nodes. Tumour size was not associated with the levels of 4-HNE. To best of our knowledge, this is the first study to use immunohistochemistry to examine the expression of 4-HNE as a prognostic factor in CC. The 3 score systems showed similar results. The pattern of 4-HNE histological appearance is dependent on the histological origin of cancer and is not universal.