TISIGNER.com: web services for improving recombinant protein production

Abstract Experiments that are planned using accurate prediction algorithms will mitigate failures in recombinant protein production. We have developed TISIGNER (https://tisigner.com) with the aim of addressing technical challenges to recombinant protein production. We offer three web services, TIsig...

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Bibliographic Details
Published inNucleic acids research Vol. 49; no. W1; pp. W654 - W661
Main Authors Bhandari, Bikash K, Lim, Chun Shen, Gardner, Paul P
Format Journal Article
LanguageEnglish
Published England Oxford University Press 02.07.2021
Subjects
Internet
Peptide Chain Initiation, Translational
Protein Sorting Signals
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Software
Solubility
Web Server Issue
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Summary:Abstract Experiments that are planned using accurate prediction algorithms will mitigate failures in recombinant protein production. We have developed TISIGNER (https://tisigner.com) with the aim of addressing technical challenges to recombinant protein production. We offer three web services, TIsigner (Translation Initiation coding region designer), SoDoPE (Soluble Domain for Protein Expression) and Razor, which are specialised in synonymous optimisation of recombinant protein expression, solubility and signal peptide analysis, respectively. Importantly, TIsigner, SoDoPE and Razor are linked, which allows users to switch between the tools when optimising genes of interest. Graphical Abstract Graphical Abstract Optimising recombinant protein production using TISIGNER.com
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The authors wish it to be known that, in their opinion, the first two authors should be regarded as Joint First Authors.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/gkab175