MiRNA profile in esophageal squamous cell carcinoma：Downregulation of miR-143 and miR-145

• Published in: World journal of gastroenterology : WJG Vol. 17; no. 1; pp. 79 - 88
• Main Authors: Wu, Bing-Li, Xu, Li-Yan, Du, Ze-Peng, Liao, Lian-Di, Zhang, Hai-Feng, Huang, Qiao, Fang, Guo-Qiang, Li, En-Min
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 07.01.2011
• Subjects: Adult; Aged; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - pathology; Carrier Proteins - genetics; Cell Line, Tumor; Cell Movement - physiology; Down-Regulation; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Female; Gene Expression Profiling; Humans; Male; Microfilament Proteins - genetics; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; miRNA; Oligonucleotide Array Sequence Analysis; Original; TaqMan; Transfection; 个人资料; 聚合酶链反应; 蛋白质水平; 表达谱芯片; 食管癌细胞; 鳞状细胞癌; Tumor invasion depth; Esophageal squamous cell carcinoma; MicroRNA; miR-143; miR-145
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v17.i1.79

AIM：To investigate the expression profile of miRNA in esophageal squamous cell carcinoma（ESCC）.METHODS：The expression profile of miRNA in ESCC tissues was analyzed by miRNA microarray.The expression levels of miR-143 and miR-145 in 86 ESCC patients were determined by real-time polymerase chain reaction（PCR） using TaqMan assay.The mobility effect was estimated by wound-healing using esophageal carcinoma cells transfected with miRNA expression plasmids.RESULTS：A set of miRNAs was found to be deregulated in the ESCC tissues,and the expression levels of miR-143 and-145 were significantly decreased in most of the ESCC tissues examined.Both miR-143 and miR-145 expression correlated with tumor invasion depth.The transfection of human esophageal carcinoma cells with miR-143 and miR-145 expression plasmids resulted in a greater inhibition of cell mobility,however,the protein level of the previously reported target of miR-145,FSCN1,did not show any significant downregulation.CONCLUSION：These findings suggest that the deregulation of miRNAs plays an important role in the progression of ESCC.Both miR-143 and miR-145 might act as anti-oncomirs common to ESCC.