Promoters on in vitro pollen germination of radish and Brassica campestris

Various media were tested for in vitro germination of pollen grains of radish and Brassica campestris. The basal medium contained 50 mg. liter-l H3BO3, 100 mg. liter-l CaCl2 and the pH was adjusted to pH 5.8. Species of pollen grains tested were: radish (Raphanus sativus L.) 'Harumaki Minowase&...

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Published inEngei Gakkai zasshi Vol. 68; no. 2; pp. 421 - 427
Main Authors Matsubara, S. (Okayama Univ. (Japan). Faculty of Agriculture), Murakami, K, Tawara, H, Hamamoto, Y, Harasawa, N
Format Journal Article
LanguageEnglish
Japanese
Published THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 1999
Subjects
BRASSICA CAMPESTRIS
CULTIVO IN VITRO
CULTURE IN VITRO
GERMINACION
GERMINATION
IN VITRO CULTURE
POLEN
POLLEN
pollen germination
promoter of pollen germination
radish pollen
surfactant
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Summary:Various media were tested for in vitro germination of pollen grains of radish and Brassica campestris. The basal medium contained 50 mg. liter-l H3BO3, 100 mg. liter-l CaCl2 and the pH was adjusted to pH 5.8. Species of pollen grains tested were: radish (Raphanus sativus L.) 'Harumaki Minowase', 'Ryujin Miura', 'Motohashikei Taibyou Minowase', and four of Brassica campestris L. 'Hiroshimana' and 'Santousai' (non-heading chinese cabbage, pekinensis group), 'Mibuna' (japonica group), and Pak-choi 'Chingensai' (chinensis group). The results obtained were as follows; 1. The basal medium containing 0.43 M sucrose yielded the highest germination rate of radish pollen compared to that at 0.29 or 0.58 M sucrose. 2. Among the media adjusted between pH 5.8 to 12.0, pH 9.0 gave the best pollen germination. 3. Germination rate was improved by using buffer solutions of pH 8.0 and 8.5, such as Taps, Tris, MES, boric acid or phosphoric acid buffer compared to distilled water. Taps and Tris buffers were most effective, followed by boric acid and barbital buffers. 4. Cholic acid, cetylmethyl ammonium bromide and sodium lauryl sulfate promoted pollen germination of radish. 5. Pollen grains of mother plants of the same age grown under 10 to 25 degree C during May to June, and October to December had the best germination rate, whereas those obtained between July to September had the poorest rate
Bibliography:F63
1999003040
ISSN:0013-7626
1880-358X
DOI:10.2503/jjshs.68.421