Phenol biosensor based on Sonogel-Carbon transducer with tyrosinase alumina sol–gel immobilization

• Published in: Analytica chimica acta Vol. 612; no. 2; pp. 198 - 203
• Main Authors: Zejli, H., Hidalgo-Hidalgo de Cisneros, J.L., Naranjo-Rodriguez, I., Liu, Baohong, Temsamani, K.R., Marty, J.L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.04.2008; Elsevier
• Subjects: Agaricales - enzymology; Alumina sol–gel; Aluminum Oxide - chemistry; Biological and medical sciences; Biosensing Techniques - methods; Biosensors; Biotechnology; Carbon - chemistry; Enzymes, Immobilized - metabolism; Fundamental and applied biological sciences. Psychology; Gels; Hydrogen-Ion Concentration; Methods. Procedures. Technologies; Microscopy, Electron, Scanning; Monophenol Monooxygenase - metabolism; Phenolic compounds; Phenols - chemistry; Reproducibility of Results; Sensitivity and Specificity; Solutions; Sonogel-Carbon electrode; Transducers; Tyrosinase; Various methods and equipments; Biosensors; Sonogel-Carbon electrode; Phenolic compounds; Tyrosinase; Alumina sol–gel; Stability; Enzyme; Carbon electrode; Immobilization; Carbon; Precursor; Reference electrode; Biosensors,Sonogel-Carbon electrode,Tyrosinase,Alumina sol-gel,Phenolic compounds; Transducer; Phenol; Sol gel process; Biosensor; Detection limit; Alumina; Ultrasound
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2008.02.029

A new biosensor for detection of phenols, based on tyrosinase immobilization with alumina sol–gel on Sonogel-Carbon transducer, has been developed. The electrode was prepared using high energy ultrasounds directly applied to the precursors. The alumina sol–gel provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenols are oxidized by tyrosinase biosensor to form a detectable product, which was determined at −300mV vs. Ag/AgCl reference electrode. For phenol, the sensor exhibited a fast response which resulted from the porous structure and high enzyme loading of the sol–gel matrix. The linear range was from 5×10−7M to 3×10−5M, with a detection limit of 3×10−7M. The stability of the biosensor was also evaluated.