Salimicrobium salexigens sp. nov., a moderately halophilic bacterium from salted hides

• Published in: Systematic and applied microbiology Vol. 34; no. 6; pp. 435 - 439
• Main Authors: de la Haba, Rafael R., Yilmaz, Pinar, Sánchez-Porro, Cristina, Birbir, Meral, Ventosa, Antonio
• Format: Journal Article
• Language: English
• Published: München Elsevier GmbH 01.09.2011; Elsevier
• Subjects: Animals; Animals, Domestic - microbiology; Australia; Bacillaceae - classification; Bacillaceae - genetics; Bacteriology; Base Sequence; Biological and medical sciences; DNA, Bacterial; Firmicutes; Fundamental and applied biological sciences. Psychology; Microbiology; Moderately halophilic bacteria; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; Salimicrobium; Salimicrobium salexigens; Salted hides; Skin - microbiology; Systematics; Australia; Moderately halophilic bacteria; Salted hides; Salimicrobium; Salimicrobium salexigens; Firmicutes; Salt; Halophily; Taxonomy; Bacteria; New species
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2011.04.002

Two Gram-positive, moderately halophilic bacteria, designated strains 29CMI T and 53CMI, were isolated from salted hides. Both strains were non-motile, strictly aerobic cocci, growing in the presence of 3–25% (w/v) NaCl (optimal growth at 7.5–12.5% [w/v] NaCl), between pH 5.0 and 10.0 (optimal growth at pH 7.5) and at temperatures between 15 and 40 °C (optimal growth at 37 °C). Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that both strains showed a similarity of 98.7% and were closely related to species of the genus Salimicrobium, within the phylum Firmicutes. Strains 29CMI T and 53CMI exhibited 16S rRNA gene sequence similarity values of 97.9–97.6% with Salimicrobium album DSM 20748 T, Salimicrobium halophilum DSM 4771 T, Salimicrobium flavidum ISL-25 T and Salimicrobium luteum BY-5 T. The DNA G+C content was 50.7 mol% and 51.5 mol% for strains 29CMI T and 53CMI, respectively. The DNA–DNA hybridization between both strains was 98%, whereas the values between strain 29CMI T and the species S. album CCM 3517 T, S. luteum BY-5 T, S. flavidum ISL-25 T and S. halophilum CCM 4074 T were 45%, 28%, 15% and 10%, respectively, showing unequivocally that strains 29CMI T and 53CMI constitute a new genospecies. The major cellular fatty acids were anteiso-C 15:0, anteiso-C 17:0, iso-C 15:0 and iso-C 14:0. The main respiratory isoprenoid quinone was MK-7, although small amounts of MK-6 were also found. The polar lipids of the type strain consist of diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one glycolipid. The peptidoglycan type is A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of the phylogenetic analysis, and phenotypic, genotypic and chemotaxonomic characteristics, we propose strains 29CMI T and 53CMI as a novel species of the genus Salimicrobium, with the name Salimicrobium salexigens sp. nov. The type strain is 29CMI T (=CECT 7568 T = JCM 16414 T = LMG 25386 T).