Nitrogen absorption, translocation and distribution from urea applied in autumn to leaves of young potted apple (Malus domestica) trees

• Published in: Tree physiology Vol. 22; no. 18; pp. 1305 - 1310
• Main Authors: Dong, Shufu, Cheng, Lailiang, Scagel, Carolyn F, Fuchigami, Leslie H
• Format: Journal Article
• Language: English
• Published: Canada 01.12.2002
• Subjects: Absorption; Amino Acids - analysis; Malus - chemistry; Malus - metabolism; Malus - physiology; Nitrogen - analysis; Nitrogen - metabolism; Plant Bark - chemistry; Plant Leaves - chemistry; Plant Leaves - metabolism; Plant Leaves - physiology; Plant Roots - chemistry; Seasons; Trees - chemistry; Trees - metabolism; Trees - physiology; Urea - metabolism
• ISSN: 0829-318X; 1758-4469
• DOI: 10.1093/treephys/22.18.1305

We studied the absorption, assimilation, translocation and distribution of nitrogen (N) from urea applied in autumn to leaves of 1-year-old potted Fuji/M26 apple (Malus domestica Borkh) trees. In early October, all leaves of each tree were painted with either 3% urea (enriched to 10 atom % with 15N) or water (control trees). Four trees were harvested before the treatment and N and amino acid contents were determined. Four trees from each treatment were harvested at 2, 4, 7, 10, 15 and 20 days after urea or water application. Total N, amino acids and 15N in leaves, bark, xylem, shank and roots were analyzed to determine uptake and mobilization of N from urea. Most uptake of 15N by leaves occurred during the first 2 days following application of urea. The mean rate of absorption during these 2 days was 0.29 g m-2 day-1. Amino acids in leaves, bark and roots increased significantly after urea application compared with control values. The highest concentrations of amino acids in leaves and bark occurred 4 days after application, whereas the highest concentrations of amino acids in roots occurred 10 days after application. Total 15N content in leaves peaked 2 days after urea application and then decreased, whereas 15N content in roots and bark increased throughout the experiment. Total 15N content in xylem and shank was low. Leaves absorbed 35% of the 15N applied as urea, and 63.6% of absorbed 15N was translocated out of leaves within 20 days after urea application. We conclude that N from urea was converted to amino acids in leaves after foliar application in autumn, and roots and bark were the main sinks of N from urea applied to leaves.