Characterisation of type-I thionin loci from the A, B, D and R genomes of wheat and rye

• Published in: Theoretical and applied genetics Vol. 96; no. 1; pp. 80 - 86
• Main Authors: Campenhout, S. van, Sagi, L, Stappen, J. vander, Volckaert, G
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 1998; Berlin Springer Nature B.V
• Subjects: amino acid sequences; Biological and medical sciences; chromosomes; Classical genetics, quantitative genetics, hybrids; DNA; DNA primers; Fundamental and applied biological sciences. Psychology; genbank/x96445; genbank/x96446; genbank/x96448; genbank/x96449; gene location; Genetic aspects; Genetic variation; Genetics; Genetics of eukaryotes. Biological and molecular evolution; genome; Genomes; Genomics; Identification and classification; loci; molecular sequence data; nucleotide sequences; Phylogeny; Plant chromosomes; Plant genetics; plant proteins; polymerase chain reaction; Pteridophyta, spermatophyta; pur-1 loci; purothionins; Secale cereale; species differences; Triticum; Triticum aestivum; Vegetals; Wheat; United Kingdom; Genetic mapping; Monocotyledones; Secale cereale; Gene; Nucleotide sequence; Gramineae; Angiospermae; Chromosome; Homology; Spermatophyta; Cereal crop; Triticum aestivum
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050712

DNA sequences encoding type-I thionins were isolated from Triticum aestivum L. cv 'Chinese Spring' using PCR with consensus primers. Blunt-end cloning, sequencing and PCR-based chromosome assignment of these fragments uncovered the three orthologous sequences corresponding to the single-copy genes at the Pur-1 loci on each of the group-1 chromosomes. Comparison with two previously published cDNA sequences revealed the presence of two introns that contain most of the polymorphic nucleotide sites. The observed orthologous DNA sequence variation among Pur-1 loci, encoded by each of the A, B and D genomes, enabled us to establish interlocus relationships and to construct locus-specific primer sets. Analogously, the Pur-R1 sequence from rye was isolated, and a locus-specific primer pair was constructed as well. Hence, four locus-specific primer sets are now available as molecular markers for the homoeologous 1AL, 1BL, 1DL and 1RL chromosome arms. Amplification from several diploid and tetraploid wheat species showed that the primers can be used as molecular tools for studying wheat phylogeny.