Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus

• Published in: Virology (New York, N.Y.) Vol. 127; no. 1; pp. 37 - 44
• Main Authors: Jackson, A.O., Dawson, J.R.O., Covey, S.N., Hull, R., Davies, J.W., Mcfarland, Janis E., Gustafson, G.D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1983
• Subjects: barley stripe mosaic virus
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/0042-6822(83)90368-9

A subgenomic (SG) RNA (∼800 nucleotides) is a minor component of barley stripe mosaic virus RNAs. The SG-RNAs isolated from the Type and North Dakota 18 (ND18) strains of BSMV have sequence homology with RNA 2 of the (“ pseudo-two component”) Type strain, which has two electrophoretic components, but only limited homology is evident with RNA 2 of the ND18 and Norwich strains, which have three electrophoretic components (“ three component” strain). Instead, eDNAs from SG-RNA hybridize most efficiently with RNA 3 of the ND18 and Norwich strains. In wheat germ extracts the SG-RNAs direct the synthesis of two polypeptides with apparent molecular weights of 20 to 21 × 10 3. However, these two polypeptides were difficult to detect by polyacrylamide gel electrophoresis of extracts from Type- or ND18-infected barley and so appear not to accumulate during infection.