Haloterrigena salina sp. nov., an extremely halophilic archaeon isolated from a salt lake

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 12; pp. 2880 - 2884
• Main Authors: Gutierrez, M. C, Castillo, A. M, Kamekura, M, Ventosa, A
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.12.2008; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Halobacteriaceae - chemistry; Halobacteriaceae - classification; Halobacteriaceae - genetics; Halobacteriaceae - physiology; Microbiology; Miscellaneous; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S - genetics; Salts; Species Specificity; Systematics; Water Microbiology; Haloterrigena; Salt lake; Archaeobacteria; Halophily; Taxonomy; Bacteria; New species; Extreme environment
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.2008/001602-0

1 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain 2 Halophiles Research Institute, 677-1 Shimizu, Noda-shi, Chiba-ken 278-0043, Japan Correspondence M. C. Gutiérrez mcg{at}us.es A novel extremely halophilic strain, designated XH-65 T , isolated from the salt lake Xilinhot in Inner Mongolia, PR China, was subjected to a polyphasic taxonomic characterization. Strain XH-65 T is neutrophilic, non-motile and requires at least 2.5 M NaCl for growth, with an optimum at 3.4 M NaCl, and grows at pH 6.0–9.0, with optimum growth at pH 7.5. Strain XH-65 T grows at 25–50 °C, with optimal growth at 37 °C. Magnesium is not required for growth. On the basis of 16S rRNA gene sequence analysis, strain XH-65 T was shown to belong to the genus Haloterrigena and was related to Haloterrigena turkmenica VKM B-1734 T (98.1 % sequence similarity), Haloterrigena saccharevitans AB14 T (96.9 %), Haloterrigena thermotolerans PR5 T (96.3 %), Haloterrigena limicola AX-7 T (95.8 %) and Haloterrigena hispanica FP1 T (95.7 %). DNA–DNA hybridization revealed 37 % relatedness between strain XH-65 T and Htg. turkmenica VKM B-1734 T . The polar lipid composition revealed the presence of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1 2)-glucose glycerol diether (S 2 -DGD). The results of the DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain XH-65 T from the six Haloterrigena species with validly published names. Therefore, strain XH-65 T represents a novel species, for which the name Haloterrigena salina sp. nov. is proposed, with the type strain XH-65 T (=CGMCC 1.6203 T =JCM 13891 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain XH-65 T is AM942763 . A thin-layer chromatogram of polar lipids of strain XH-65 T and related haloarchaea is available as supplementary material with the online version of this paper.