IL-4 and granulocyte-macrophage colony-stimulating factor selectively increase HLA-DR and HLA-DP antigens but not HLA-DQ antigens on human monocytes

• Published in: The Journal of immunology (1950) Vol. 144; no. 12; pp. 4670 - 4674
• Main Authors: Gerrard, TL, Dyer, DR, Mostowski, HS
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.06.1990
• Subjects: Biological Factors - pharmacology; Colony-Stimulating Factors - pharmacology; Cytokines; Dose-Response Relationship, Drug; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - pharmacology; HLA-DP Antigens - metabolism; HLA-DQ Antigens - metabolism; HLA-DR Antigens - metabolism; Humans; In Vitro Techniques; Interferon-gamma - pharmacology; Interleukin-4 - pharmacology; Monocytes - immunology; Monocytes - metabolism; Recombinant Proteins
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.144.12.4670

A number of cytokines were tested for their ability to modulate HLA-DR Ag expression on normal human monocytes. IL-4, granulocyte-macrophage (GM)-CSF as well as IFN-gamma were able to increase HLA-DR Ag expression on monocytes. IFN-alpha was also able to augment HLA-DR Ag expression, but to a lesser degree. Macrophage-CSF, granulocyte-CSF, TNF-alpha, TNF-beta, and IL-6 were not able to augment HLA-DR Ag expression. There were distinct patterns in the ability of different cytokines to augment class II histocompatibility Ag expression. IL-4 and GM-CSF selectively increased only HLA-DR and HLA-DP, but did not increase HLA-DQ antigens on monocytes. IFN-gamma, however, was able to augment the expression of HLA-DR, HLA-DP, and HLA-DQ Ag. Combinations of IFN-gamma with either IL-4 or GM-CSF did not show any synergy for the augmentation of any of the class II antigens on monocytes.