The non-steroidal anti-inflammatory drug piroxicam blocks ligand binding to the formyl peptide receptor but not the formyl peptide receptor like 1

• Published in: Biochemical pharmacology Vol. 74; no. 7; pp. 1050 - 1056
• Main Authors: Stenfeldt, A.-L., Karlsson, J., Wennerås, C., Bylund, J., Fu, H., Dahlgren, C.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2007; Elsevier Science
• Subjects: Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Biological and medical sciences; Dose-Response Relationship, Drug; Formyl peptide receptor; Formyl peptide receptor like 1; Humans; Inhibitor; Ligands; Medical sciences; Neutrophil; Neutrophils - drug effects; Non-steroidal anti-inflammatory drug; Oligopeptides - pharmacology; Pharmacology. Drug treatments; Piroxicam; Piroxicam - pharmacology; Protein Binding - drug effects; Receptors, Formyl Peptide - agonists; Receptors, Formyl Peptide - antagonists & inhibitors; Receptors, Formyl Peptide - metabolism; Receptors, Lipoxin - antagonists & inhibitors; Receptors, Lipoxin - metabolism; Superoxides - metabolism; Piroxicam; Inhibitor; Neutrophil; Formyl peptide receptor like 1; Non-steroidal anti-inflammatory drug; Formyl peptide receptor; Prostaglandin-endoperoxide synthase; Ligand binding; Oxicam derivatives; Enzyme; Granulocyte; Enzyme inhibitor; fMLP receptor; Non steroidal antiinflammatory agent; Analgesic; G protein coupled receptor; Antipyretic; Oxidoreductases
• ISSN: 0006-2952; 1873-2968
• DOI: 10.1016/j.bcp.2007.06.049

The anti-inflammatory drug piroxicam has been reported to affect the production of reactive oxygen species in phagocytes. This anti-inflammatory effect is thought to be mediated through inhibition of cyclooxygenase (COX), an enzyme important for prostaglandin synthesis. We have compared the effects of piroxicam on superoxide production mediated by two closely related G-protein coupled receptors expressed on neutrophils, the formyl peptide receptor (FPR) and the formyl peptide receptor like 1 (FPRL1). Neutrophils were stimulated with agonists that bind specifically to FPR (the peptide ligand N-formyl-Met-Leu-Phe, fMLF) or FPRL1 (the peptide ligand Trp-Lys-Tyr-Met-Val-L-Met-NH2, WKYMVM) or both of these receptors (the peptide ligand Trp-Lys-Tyr-Met-Val-D-Met-NH2, WKYMVm). Piroxicam reduced the neutrophil superoxide production induced by the FPR agonist but had no significant effect on the FPRL1 induced response. Neutrophil intracellular calcium changes induced by the agonist WKYMVm (that triggers both FPR and FPRL1) were only inhibited by piroxicam when the drug was combined with the FPRL1 specific antagonist, Trp-Arg-Trp-Trp-Trp-Trp (WRW4), and this was true also for the inhibition of superoxide anion release. Receptor-binding analysis showed that the fluorescently labelled FPR specific ligand N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys (fNLFNYK), was competed for in a dose-dependent manner, by the FPR ligand fMLF and as well as by piroxicam. We show that piroxicam inhibits the neutrophil responses triggered through FPR, but not through FPRL1 and this inhibition is due to a reduced binding of the activating ligand to its cell surface receptor.