Modulatory effects of sesamol in dinitrochlorobenzene-induced inflammatory bowel disorder in albino rats

• Published in: Pharmacological reports Vol. 65; no. 3; pp. 658 - 665
• Main Authors: Kondamudi, Phani Krishna, Kovelamudi, Hemalatha, Mathew, Geetha, Nayak, Pawan G., Rao, C. Mallikarjuna, Shenoy, Rekha R.
• Format: Journal Article
• Language: English
• Published: Cham Elsevier B.V 2013; Springer International Publishing
• Subjects: Animals; Antioxidants - pharmacology; Benzodioxoles - pharmacology; Body Weight - drug effects; Colon; dinitrochlorobenzene; Dinitrochlorobenzene - adverse effects; Drug Safety and Pharmacovigilance; Granulocyte Colony-Stimulating Factor - metabolism; Inflammatory Bowel Diseases - blood; Inflammatory Bowel Diseases - chemically induced; Inflammatory Bowel Diseases - drug therapy; Inflammatory Bowel Diseases - metabolism; Interleukin-3 - metabolism; interleukin-6; Interleukin-6 - metabolism; Male; myeloperoxidase; Nitrites - blood; Nitrites - metabolism; Pharmacotherapy; Pharmacy; Phenols - pharmacology; Rats; Rats, Wistar; Recombinant Fusion Proteins - metabolism; sesamol; TBARS; Thiobarbituric Acid Reactive Substances - metabolism; TNF-α; Tumor Necrosis Factor-alpha - metabolism; SES; SS; dinitrochlorobenzene; CD; sesamol; IL; DNCB; myeloperoxidase; TBARS; IBD; MPO; TNF; interleukin-6; UC; TNF-α; ROS
• ISSN: 1734-1140; 2299-5684
• DOI: 10.1016/S1734-1140(13)71043-0

Inflammatory bowel disease (IBD) is a chronic inflammatory condition of gastrointestinal tract of immune, genetic and environmental origin. In the present study, we examined the effect of sesamol (SES), the main anti-oxidative constituent of Sesamum indicum (sesame seed) Linn. in the dinitrochlorobenzene (DNCB)-induced model for IBD in rats. The groups were divided into normal control, DNCB control, SES and sulfasalazine (SS). On day 24, the rats were killed, colon removed and the macroscopic, biochemical and histopathological evaluations were performed. The levels of MPO, TBARS and nitrite increased significantly (p<0.05) in the DNCB group, whereas reduced significantly in the SES, SS treated groups. Serum nitrite levels were found to be insignificant between the different groups. IL-6 and TNF-α levels were significantly high in the DNCB group. We conclude the mucosal protective effect of SES on colon due to its potent antioxidant actions. Further investigation is required in a chronic model of different rodent strain for its role involved in the cytokine pathway.