An integrated approach for the valorization of mango seed kernel: Efficient extraction solvent selection, phytochemical profiling and antiproliferative activity assessment

• Published in: Food research international Vol. 126; p. 108616
• Main Authors: Ballesteros-Vivas, Diego, Álvarez-Rivera, Gerardo, Morantes, Sandra Johanna, Sánchez-Camargo, Andrea del Pilar, Ibáñez, Elena, Parada-Alfonso, Fabián, Cifuentes, Alejandro
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.12.2019
• Subjects: Antiproliferative activity; CCD-18Co cell line; Fruit by-products; GC-Q-TOF; Hansen solubility parameters; High-resolution mass spectrometry; HT-29 cell line; LC-Q-TOF; Mangifera indica L; Pressurized-liquid extraction; Hansen solubility parameters; Pressurized-liquid extraction; GC-Q-TOF; Antiproliferative activity; Mangifera indica L; CCD-18Co cell line; LC-Q-TOF; High-resolution mass spectrometry; HT-29 cell line; Fruit by-products
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2019.108616

A novel valorization strategy is proposed in this work for the sustainable utilization of a major mango processing waste (i.e. mango seed kernel, MSK), integrating green pressurized-liquid extraction (PLE), bioactive assays and comprehensive HRMS-based phytochemical characterization to obtain bioactive-rich fractions with high antioxidant capacity and antiproliferative activity against human colon cancer cells. Thus, a two steps PLE procedure was proposed to recover first the non-polar fraction (fatty acids and lipids) and second the polar fraction (polyphenols). Efficient selection of the most suitable solvent for the second PLE step (ethanol/ethyl acetate mixture) was based on the Hansen solubility parameters (HSP) approach. A comprehensive GC- and LC-Q-TOF-MS/MS profiling analysis allowed the complete characterization of the lipidic and phenolic fractions obtained under optimal condition (100% EtOH at 150 °C), demonstrating the abundance of oleic and stearic acids, as well as bioactive xanthones, phenolic acids, flavonoids, gallate derivatives and gallotannins. The obtained MSK-extract exhibited higher antiproliferative activity against human colon adenocarcinoma cell line HT-29 compared to traditional extraction procedures described in literature for MSK utilization (e.g. Soxhlet), demonstrating the great potential of the proposed valorization strategy as a valuable opportunity for mango processing industry to deliver a value-added product to the market with health promoting properties. [Display omitted] •Powerful analytical strategy for mango seed kernel valorization•Green extraction procedure in two steps to recover lipidic and phenolic fractions•Phytochemical profiling of obtained PLE fractions by LC/GC-q-TOF-MS(/MS)•Antiproliferative activity of the phenolic extracts against colon cancer cells