Urinary polyamines and N-acetylated polyamines in four patients with Alzheimer's disease as their N-ethoxycarbonyl- N-pentafluoropropionyl derivatives by gas chromatography–mass spectrometry in selected ion monitoring mode

• Published in: Analytica chimica acta Vol. 576; no. 1; pp. 55 - 60
• Main Authors: Paik, Man-Jeong, Lee, Sunmie, Cho, Kyung-Hea, Kim, Kyoung-Rae
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 18.08.2006; Elsevier
• Subjects: Alzheimer's disease; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Ethoxycarbonylation; Exact sciences and technology; Gas chromatographic methods; GC–SIM–MS; Pentafluoropropionylation; Spectrometric and optical methods; Star pattern recognition; Urinary polyamines; Ethoxycarbonylation; Pentafluoropropionylation; Urinary polyamines; GC–SIM–MS; Alzheimer's disease; Star pattern recognition; Cadaverine; GC-SIM-MS; Polyamine; Secondary ion mass spectrometry; Pattern recognition; Gas chromatography; Simultaneous measurement; Ion spectrometry; Standard deviation; Relative error; Mass spectrometry; Monitoring
• ISSN: 0003-2670; 1873-4324; 1873-4324
• DOI: 10.1016/j.aca.2006.01.070

Simultaneous determination of putrescine, cadaverine, spermidine, spermine, N 1-acetylputrescine, N 1-acetylcadaverine, N 1-acetylspermidine, N 8-acetylspermidine and N 1-acetylspermine in aqueous samples was achieved as their N-ethoxycarbonyl- N-pentafluoropropionyl derivatives by gas chromatography–mass spectrometry (GC–MS) in selected ion monitoring (SIM) mode. The overall GC–SIM–MS method was linear ( r ≥ 0.9987), repetitive (% relative standard deviation = 1.3–6.4), and accurate (% relative error = −2.1–8.6). As compared to normal subjects, the levels of putrescine, N 1-acetylspermine and spermine were significantly elevated while the levels of N 1-acetylputrescine, N 1-acetylcadaverine and N 1-acetylspermidine were markedly reduced in all four Alzheimer's disease (AD) patients. When star symbol plotting was applied to the normalized levels of nine polyamines relative to the corresponding normal mean values, each star pattern of the AD patients was deformed, thus being readily distinguishable from the nonagon shape of the normal group average.