The bHLH142 Transcription Factor Coordinates with TDR1 to Modulate the Expression of EAT1 and Regulate Pollen Development in Rice

Male sterility plays an important role in F1 hybrid seed production. We identified a male-sterile rice (Oryza sativa) mutant with impaired pollen development and a single T-DNA insertion in the transcription factor gene bHLH142. Knockout mutants of bHLH142 exhibited retarded meiosis and defects in t...

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Published inThe Plant cell Vol. 26; no. 6; pp. 2486 - 2504
Main Authors Ko, Swee-Suak, Li, Min-Jeng, Ku, Maurice Sun-Ben, Ho, Yi-Cheng, Lin, Yi-Jyun, Chuang, Ming-Hsing, Hsing, Hong-Xian, Lien, Yi-Chen, Yang, Hui-Ting, Chang, Hung-Chia, Chan, Ming-Tsair
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Biologists 01.06.2014
Subjects
Anthers
Developmental biology
Gene expression regulation
Meiosis
Microspores
Plant cells
Plants
Pollen
Proteins
Rice
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Summary:Male sterility plays an important role in F1 hybrid seed production. We identified a male-sterile rice (Oryza sativa) mutant with impaired pollen development and a single T-DNA insertion in the transcription factor gene bHLH142. Knockout mutants of bHLH142 exhibited retarded meiosis and defects in tapetai programmed cell death. RT-PCR and in situ hybridization analyses showed that bHLH142 is specifically expressed in the anther, in the tapetum, and in meiocytes during early meiosis. Three basic helix-loop-helix transcription factors, UDT1 (bHLH164), TDR1 (bHLH5), and EAT1/DTD1 (bHLH141) are known to function in rice pollen development. bHLH142 acts downstream of UDT1 and GAMYB but upstream of TDR1 and EAT1 in pollen development. In vivo and in vitro assays demonstrated that bHLH142 and TDR1 proteins interact. Transient promoter assays demonstrated that regulation of the EAT1 promoter requires bHLH142 and TDR1. Consistent with these results, 3D protein structure modeling predicted that bHLH142 and TDR1 form a heterodimer to bind to the EAT1 promoter. EAT1 positively regulates the expression of AP37 and AP25, which induce tapetai programmed cell death. Thus, in this study, we identified bHLH142 as having a pivotal role in tapetai programmed cell death and pollen development.
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These authors contributed equally to this work.
www.plantcell.org/cgi/doi/10.1105/tpc.114.126292
The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (www.plantcell.org) is: Swee-Suak Ko (sweesuak@gate.sinica.edu.tw).
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.114.126292