Serotonin derivative, N-(p-Coumaroyl) serotonin, isolated from safflower (Carthamus tinctorius L.) oil cake augments the proliferation of normal human and mouse fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF)

• Published in: Journal of biochemistry (Tokyo) Vol. 125; no. 5; pp. 910 - 915
• Main Authors: Takii, T, Hayashi, M, Hiroma, H, Chiba, T, Kawashima, S, Zhang, H.L, Nagatsu, A, Sakakibara, J, Onozaki, K
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.1999
• Subjects: Animals; Antioxidants - pharmacology; cell division; Cell Division - drug effects; Cell Line; cell proliferation; coumaroyl serotonin; derivatives; Drug Synergism; EGF; epidermal growth factor; Epidermal Growth Factor - pharmacology; FGF; fibroblast; Fibroblast Growth Factor 1 - pharmacology; Fibroblast Growth Factor 2 - pharmacology; fibroblasts; Fibroblasts - cytology; Fibroblasts - drug effects; growth factor; Humans; inhibition; interactions; linoleic acid; lipid peroxidation; Mice; oilseed cakes; oxidation; Platelet-Derived Growth Factor - pharmacology; safflower meal; serotonin; Serotonin - isolation & purification; Serotonin - pharmacology; stimulation; Tumor Cells, Cultured
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/oxfordjournals.jbchem.a022368

N-(p-Coumaroyl)serotonin (CS) with antioxidative activity is present in safflower oil. We have reported that CS inhibits proinflammatory cytokine generation from human mono-cytes in vitro. As reactive oxygen species (ROS) affect cell proliferation, in this study the effect of CS on the proliferation of various cell types was examined. CS augments the proliferation of normal human and mouse fibroblast cells. The cells continue to proliferate in the presence of CS and form a transformed cell-like focus without transformation. CS, however, does not augment the proliferation of other cell types, either normal or tumor cells. CS augments the proliferation of fibroblasts in synergy with basic fibroblast growth factor (bFGF) or epidermal growth factor (EGF), but not with acidic FGF(aFGF) or platelet-derived growth factor (PDGF). This study using synthesized derivatives of CS reveals that the growth-promoting activity is not due to antioxidative activity. These findings indicate that CS is a natural compound with unique growth-promoting activity for fibroblasts