Proteomics Profiling of Neuron-Derived Small Extracellular Vesicles from Human Plasma: Enabling Single-Subject Analysis

• Published in: International journal of molecular sciences Vol. 22; no. 6; p. 2951
• Main Authors: Anastasi, Federica, Masciandaro, Silvia Maria, Carratore, Renata Del, Dell’Anno, Maria Teresa, Signore, Giovanni, Falleni, Alessandra, McDonnell, Liam A., Bongioanni, Paolo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.03.2021; MDPI
• Subjects: Biomarkers; Biomarkers - blood; Brain; Cell adhesion & migration; Chromatography; Chromatography, Liquid; Exosomes - metabolism; Extracellular vesicles; Extracellular Vesicles - genetics; Extracellular Vesicles - metabolism; Humans; Morphology; Neurons; Neurons - metabolism; Parkinson Disease - blood; Parkinson Disease - genetics; Parkinson Disease - pathology; Parkinson's disease; Patients; Plasma; Proteins; Proteome - genetics; Proteomics; Tandem Mass Spectrometry; NES cells; proteomics; exosomes; miR-155; neuron-derived vesicles; small extracellular vesicles; L1CAM; plasma; Parkinson’s disease; central nervous system
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22062951

Small extracellular vesicles have been intensively studied as a source of biomarkers in neurodegenerative disorders. The possibility to isolate neuron-derived small extracellular vesicles (NDsEV) from blood represents a potential window into brain pathological processes. To date, the absence of sensitive NDsEV isolation and full proteome characterization methods has meant their protein content has been underexplored, particularly for individual patients. Here, we report a rapid method based on an immunoplate covalently coated with mouse monoclonal anti-L1CAM antibody for the isolation and the proteome characterization of plasma-NDsEV from individual Parkinson’s disease (PD) patients. We isolated round-shaped vesicles with morphological characteristics consistent with exosomes. On average, 349 ± 38 protein groups were identified by liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, 20 of which are annotated in the Human Protein Atlas as being highly expressed in the brain, and 213 were shared with a reference NDsEV dataset obtained from cultured human neurons. Moreover, this approach enabled the identification of 23 proteins belonging to the Parkinson disease KEGG pathway, as well as proteins previously reported as PD circulating biomarkers.