Lipid and unsaturated fatty acid productions from three microalgae using nitrate and light-emitting diodes with complementary LED wavelength in a two-phase culture system

• Published in: Bioprocess and biosystems engineering Vol. 42; no. 9; pp. 1517 - 1526
• Main Authors: Kim, So Hee, Sunwoo, In Yung, Hong, Hee Jun, Awah, Che Clovis, Jeong, Gwi-Taek, Kim, Sung-Koo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2019; Springer Nature B.V
• Subjects: Acid production; Algae; Algal growth; Biomass; Biotechnology; Cell Culture Techniques; Chemistry; Chemistry and Materials Science; Chlorella; Chlorella vulgaris - growth & development; Culture; Docosahexaenoic acid; Eicosapentaenoic acid; Environmental Engineering/Biotechnology; Fatty Acids, Unsaturated - biosynthesis; Food Science; Haptophyta - growth & development; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Light; Light effects; Light emitting diodes; Lighting; Lipids; Microalgae; Microalgae - growth & development; Nitrates; Organic light emitting diodes; Pavlova lutheri; Porphyridium - growth & development; Porphyridium cruentum; Research Paper; Wavelength; Wavelengths; Complementary wavelength; Two-phase culture; Light-emitting diodes; Polyunsaturated fatty acids
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-019-02149-y

In this study, Pavlova lutheri, Chlorella vulgaris, and Porphyridium cruentum were cultured using modified F/2 media in a 1 L flask culture. Various nitrate concentrations were tested to determine an optimal nitrate concentration for algal growth. Subsequently, the effect of light emitted at a specific wavelength on biomass and lipid production by three microalgae was evaluated using various wavelengths of light-emitting diodes (LED). Biomass production by P. lutheri , C. vulgaris , and P. cruentum were the highest with blue, red, and green LED wavelength with 1.09 g dcw/L, 1.23 g dcw/L, and 1.28 g dcw/L on day 14, respectively. Biomass production was highest at the complementary LED wavelength to the color of microalgae. Lipid production by P. lutheri, C. vulgaris, and P. cruentum were the highest with yellow, green, and red LEDs’ wavelength, respectively. Eicosapentaenoic acid production by P. lutheri, C. vulgaris, and P. cruentum was 10.35%, 10.14%, and 14.61%, and those of docosahexaenoic acid were 6.09%, 8.95%, and 11.29%, respectively.