SNHG16 promotes cell proliferation and inhibits cell apoptosis via regulation of the miR-1303-p/STARD9 axis in clear cell renal cell carcinoma

• Published in: Cellular signalling Vol. 84; p. 110013
• Main Authors: Cheng, Tao, Shuang, Weibing, Ye, Dawen, Zhang, Wenzhi, Yang, Zhao, Fang, Wenge, Xu, Haibin, Gu, Mingli, Xu, Weiqiang, Guan, Chao
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.08.2021
• Subjects: Apoptosis - genetics; Carcinoma, Renal Cell - genetics; Carrier Proteins; Cell Proliferation - genetics; Humans; Kidney Neoplasms - genetics; MicroRNAs - genetics; MicroRNAs - metabolism; miR-1301-3p; Renal cell carcinoma; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; SNHG16; STARD9; SNHG16; miR-1301-3p; Renal cell carcinoma; STARD9
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/j.cellsig.2021.110013

Clear cell renal cell carcinoma (ccRCC) is a common subtype of renal cell carcinoma (RCC) and causes many deaths. Numerous medical studies have suggested that long noncoding RNAs (lncRNAs) exert their biological functions on ccRCC. Herein, functions of lncRNA SNHG16 in ccRCC cells and the mechanism mediated by SNHG16 were investigated. The expression levels of SNHG16 and its downstream genes in ccRCC cells and RCC tissues were examined utilizing reverse transcription quantitative polymerase chain reaction analyses. Cell counting kit-8 and 5-Ethynyl-2′-deoxyuridine assays were performed to evaluate the proliferation of ccRCC cells, and flow cytometry analyses were employed to determine the apoptosis of ccRCC cells. Western blot analysis was applied to examine protein levels associated with cell proliferation and apoptosis. The combination between SNHG16 and miRNA as well as miRNA and its target gene were explored by luciferase reporter, RNA pull down, and RNA immunoprecipitation assays. The significant upregulation of SNHG16 was observed in RCC tissues and ccRCC cells. SNHG16 downregulation inhibited the proliferation and promoted the apoptosis of ccRCC cells. In addition, SNHG16 served as a competing endogenous RNA for miR-1301-3p, and STARD9 was a target gene of miR-1301-3p in ccRCC cells. SNHG16 upregulated STARD9 expression by binding with miR-1301-3p in ccRCC cells. Rescue assays validated that SNHG16 promoted ccRCC cell promotion and induced ccRCC cell apoptosis by upregulating STARD9 expression. In conclusions, SNHG16 promotes ccRCC cell proliferation and suppresses ccRCC cell apoptosis via interaction with miR-1301-3p to upregulate STARD9 expression in ccRCC cells. •SNHG16 exhibits high expression in RCC cells and tissues.•SNHG16 knockdown inhibits RCC cell proliferation and promotes cell apoptosis.•SNHG16 combines with miR-1301-3p to upregulate STARD9 expression.•STARD9 reverses SNHG16 depletion-induced effects on cell proliferation and apoptosis.