Luteolin alters MUC1 extracellular domain, sT antigen, ADAM-17, IL-8, IL-10 and NF-κB expression in Helicobacter pylori -infected gastric cancer CRL-1739 cells: A preliminary study

• Published in: Biomedical reports Vol. 14; no. 2; p. 19
• Main Authors: Radziejewska, Iwona, Borzym-Kluczyk, Małgorzata, Leszczyńska, Katarzyna
• Format: Journal Article
• Language: English
• Published: England Spandidos Publications UK Ltd 01.02.2021; D.A. Spandidos
• Subjects: Antibodies; Anticancer properties; Antigens; Antiinfectives and antibacterials; Antioxidants; Bacteria; Chronic infection; Domains; Flavonoids; Gastric cancer; Gastritis; Gene expression; Helicobacter pylori; Infections; Inflammation; Interleukin 10; Interleukin 8; Laboratories; Lectins; Metastasis; Microorganisms; NF-κB protein; Oxidants; Oxidizing agents; Pathogenesis; Proteins; Reverse transcription; Transcription factors; Poland; NF-κB; luteolin; MUC1; Helicobacter pylori; gastric cancer; interleukin
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2020.1395

Luteolin is a natural flavonoid possessing certain beneficial pharmacological properties, including anti-oxidant, anti-inflammatory, anti-microbial and anti-cancer properties. The majority of types of gastric cancer with chronic gastritis are caused by infection with ( ). The present study evaluated the effect of luteolin on a number of selected factors that are potentially involved in gastric cancer development. The study was performed using gastric cancer CRL-1739 cells treated with 30 µM luteolin and alone or combined. ELISA and reverse transcription PCR were used to assess the expression levels of MUC1, GalNAcα-R (Tn antigen) and NeuAcα2-3Galβ1-3GalNAc-R (sT antigen), ADAM-17, IL-8, IL-10 and NF-κB. and luteolin independently and in combination significantly reduced the expression levels of the extracellular domain of MUC1 in gastric cancer cells compared with the untreated control cells. ADAM-17 expression was reduced by treatment with the pathogen and luteolin. Additionally, both factors reduced sT antigen expression. Treatment with 30 ≤M luteolin significantly induced IL-8 expression at the mRNA and protein level, and the mRNA expression levels of IL-10 and NF-κB compared with the control. Both and luteolin induced IL-8 protein expression. The present preliminary results suggest that luteolin may be used to treat patients with gastric cancer.