Visualization of yeast single-cells on fabric surface with a fluorescent glucose and their isolation for culture

• Published in: Journal of industrial microbiology & biotechnology Vol. 34; no. 10; pp. 685 - 688
• Main Authors: Fujioka, Kohtaro, Geis, Philip, Saito, Mikako, Matsuoka, Hideaki
• Format: Journal Article
• Language: English
• Published: Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2007; Springer; Springer Nature B.V
• Subjects: 4-Chloro-7-nitrobenzofurazan - analogs & derivatives; 4-Chloro-7-nitrobenzofurazan - metabolism; Bacteria; Biological and medical sciences; Biotechnology; Candida albicans; Candida albicans - cytology; Candida albicans - metabolism; Cotton Fiber; Deoxyglucose - analogs & derivatives; Deoxyglucose - metabolism; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - chemistry; Glucose - metabolism; Identification systems; Infections; Microbiological Techniques; Microorganisms; Microscopy, Fluorescence - methods; Surface Properties; Textiles - microbiology; Yeasts; Visualization; Yeast; Ultra-deep focusing range (UDF) fluorescent microscope Single-cell manipulation Fluorescent glucose derivative Viable cell imaging; Imaging; Isolated cell; Fluorescence; Isolation; Glucose; Imagery; Viability; Culture
• ISSN: 1367-5435; 1476-5535
• DOI: 10.1007/s10295-007-0231-7

An ultra-deep focusing range (UDF) fluorescent microscope system has been combined with a micromanipulation system to develop a viable cell detection-identification system applicable to microbes on environmental surfaces and products. Candida albicans yeast cells on a fabric sample surface were viably stained with a fluorescent glucose derivative, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy glucose (2-NBDG) and detected with a UDF fluorescent microscope. Visualized single-cells of C. albicans were picked in a glass microcapillary and transferred onto an agar medium. After the culture, the colony was assayed for DNA sequence to identify the isolate. This demonstrates a potential application to the study of unknown environmental microorganisms.