Mesophilic anaerobic digestion of pig slurry and fruit and vegetable waste: Dissection of the microbial community structure

This paper studies the abiotic properties and the microbial community of a pilot scale digester with a working volume capacity of 300 L. The system worked at mesophilic operating conditions, with several organic load rates formed by a mixture of different ratios between waste A (fruit and vegetable...

Full description

Saved in:
Bibliographic Details
Published inJournal of cleaner production Vol. 156; pp. 757 - 765
Main Authors Ros, Margarita, de Souza Oliveira Filho, Jose, Perez Murcia, Maria Dolores, Bustamante, Maria Angeles, Moral, Raul, Coll, Maria Dolores, Lopez Santisima-Trinidad, Ana Belen, Pascual, Jose Antonio
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 10.07.2017
Subjects
Biogas
Clostridium
Fruit and vegetable sludge
Pig slurry
Tomato wastes
Clostridium
TAN
FVS
AD
TW
PS
C/N
Pig slurry
VFA/TA ratio
TOC
Biogas
Fruit and vegetable sludge
Tomato wastes
FAN
OLR
TN
VS
EC
TS
Online AccessGet full text

Cover

More Information
Summary:This paper studies the abiotic properties and the microbial community of a pilot scale digester with a working volume capacity of 300 L. The system worked at mesophilic operating conditions, with several organic load rates formed by a mixture of different ratios between waste A (fruit and vegetable sludge + tomato waste) and waste B (pig slurry). The core group of microorganisms corresponding to the ratio 70% A:30% B, showed the highest methane production (60%). This core group included bacteria, principally Firmicutes and Bacteroidetes, involved in cellulolytic degradation, proteolysis and acidogenesis, as well as a concomitance between acetotrophic, mainly Methanosaeta methanogens and hydrogenotrophic, principally Methanosphaera and Methanobrevibacter methanogens. An increase of the proportion of waste B (pig slurry) to 50–60% resulted in a decrease of the production of biogas and methane, which unbalanced the methanogenic bacteria, increasing the amount of principally hydrogenotropic methanogens (Methanosphaera and Methanobrevibacter). [Display omitted] •An adequate organic-feedstock for AD was the ratio (FVW + TW) 70%/(PS) 30%.•The highest methane production included principally Firmicutes and Bacteroidetes.•The highest methanogens involved Methanosaeta, Methanosphaera, Methanobrevibacter.
ISSN:0959-6526
1879-1786
DOI:10.1016/j.jclepro.2017.04.110