Engineering of glycerol utilization in Pseudomonas chlororaphis GP72 for enhancing phenazine-1-carboxylic acid production

• Published in: World journal of microbiology & biotechnology Vol. 36; no. 3; p. 49
• Main Authors: Song, Chen, Yue, Sheng-Jie, Liu, Wen-Hui, Zheng, Yi-Fan, Zhang, Cheng-Hao, Feng, Tong-Tong, Hu, Hong-Bo, Wang, Wei, Zhang, Xue-Hong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 10.03.2020; Springer Nature B.V
• Subjects: Acid production; Applied Microbiology; Aquaporins - genetics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biochemistry; Biodiesel fuels; Biofuels; Biomedical and Life Sciences; Biotechnology; Blight; Capsicum - microbiology; Carboxylic acids; China; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Environmental Engineering/Biotechnology; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes; Glycerol; Glycerol - metabolism; Glycerolphosphate Dehydrogenase - genetics; Life Sciences; Metabolic Engineering - methods; Metabolic Networks and Pathways - genetics; Metabolism; Microbiology; Original Paper; Phenazine; Phenazine-1-carboxylic acid; Phenazines - metabolism; Plant growth; Pseudomonas; Pseudomonas chlororaphis; Pseudomonas chlororaphis - genetics; Pseudomonas chlororaphis - metabolism; Repressor Proteins - genetics; Rhizosphere; Sheath blight; Sheaths; Synthesis; Utilization; China; High value-added compounds; Phenazine-1-carboxylic acid; Biosynthesis; Glycerol utilization; GP72; Pseudomonas chlororaphis GP72
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-020-02824-3

Glycerol is a by-product of biodiesel, and it has a great application prospect to be transformed to synthesize high value-added compounds. Pseudomonas chlororaphis GP72 isolated from the green pepper rhizosphere is a plant growth promoting rhizobacteria that can utilize amount of glycerol to synthesize phenazine-1-carboxylic acid (PCA). PCA has been commercially registered as “Shenqinmycin” in China due to its characteristics of preventing pepper blight and rice sheath blight. The aim of this study was to engineer glycerol utilization pathway in P. chlororaphis GP72. First, the two genes glpF and glpK from the glycerol metabolism pathway were overexpressed in GP72ANO separately. Then, the two genes were co-expressed in GP72ANO, improving PCA production from 729.4 mg/L to 993.4 mg/L at 36 h. Moreover, the shunt pathway was blocked to enhance glycerol utilization, resulting in 1493.3 mg/L PCA production. Additionally, we confirmed the inhibition of glpR on glycerol metabolism pathway in P . chlororaphis GP72. This study provides a good example for improving the utilization of glycerol to synthesize high value-added compounds in Pseudomonas .