Protein kinase Cα suppresses the expression of STC1 in MDA-MB-231 breast cancer cells

• Published in: Tumor biology Vol. 32; no. 5; pp. 1023 - 1030
• Main Authors: Cornmark, Louise, Lønne, Gry Kalstad, Jögi, Annika, Larsson, Christer
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.10.2011
• Subjects: Biomedical and Life Sciences; Biomedicine; Blotting, Western; Breast cancer cells; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Cancer and Oncology; Cancer och onkologi; Cancer Research; Cell Line, Tumor; Clinical Medicine; Female; Gene Expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - physiology; Glycoproteins - biosynthesis; Glycoproteins - genetics; Humans; Klinisk medicin; Medical and Health Sciences; Medicin och hälsovetenskap; Microarray Analysis; Protein kinase C; Protein Kinase C-alpha - genetics; Protein Kinase C-alpha - metabolism; Research Article; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - analysis; RNA, Small Interfering; Stanniocalcin 1; Transfection; Protein kinase C; Gene expression; Breast cancer cells; Stanniocalcin 1
• ISSN: 1010-4283; 1423-0380; 1423-0380
• DOI: 10.1007/s13277-011-0205-2

Several protein kinase C (PKC) isoforms have been shown to influence different cellular processes that may contribute to the malignancy of breast cancer cells. To obtain insight into mechanisms mediating the PKC effects, global gene expression was analyzed in MDA-MB-231 breast cancer cells in which PKCα, PKCδ or PKCε had been down-regulated with siRNA. Gene set enrichment analyses revealed that hypoxia-induced genes were enriched among genes that increased in PKCα-down-regulated cells. The STC1 mRNA, encoding stanniocalcin 1, was particularly up-regulated following depletion of PKCα and was also induced by hypoxia. Both hypoxia and PKCα down-regulation also led to increased STC1 protein levels. The results demonstrate that PKCα suppresses the expression of STC1 in breast cancer cells.