Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells

• Published in: World journal of gastroenterology : WJG Vol. 9; no. 3; pp. 454 - 458
• Main Authors: Li, Qi-Fu, Ou-Yang, Gao-Liang, Peng, Xuan-Xian, Hong, Shui-Gen
• Format: Journal Article
• Language: English
• Published: United States The Key Laboratory of China Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China%Laboratory of Cell Biology, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China 01.03.2003; Baishideng Publishing Group Inc
• Subjects: Antimicrobial Cationic Peptides; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Cycle - drug effects; DNA-Binding Proteins - pharmacology; Humans; Immunohistochemistry; Liver Cancer; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Peptides, Cyclic - pharmacology; Tumor Cells, Cultured
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v9.i3.454

To investigate the effects of tachyplesin on the cell cycle regulation in human hepatcarcinoma cells. Effects of tachyplesin on the cell cycle in human hepatocarcinoma SMMC-7721 cells were assayed with flow cytometry. The protein levels of p53, p16, cyclin D1 and CDK4 were assayed by immunocytochemistry. The mRNA levels of p21(WAF1/CIP1) and c-myc genes were examined with in situ hybridization assay. After tachyplesin treatment, the cell cycle arrested at G0/G1 phase, the protein levels of mutant p53, cyclin D1 and CDK4 and the mRNA level of c-myc gene were decreased, whereas the levels of p16 protein and p21(WAF1/CIP1) mRNA increased. Tachyplesin might arrest the cell at G0/G1 phase by upregulating the levels of p16 protein and p21(WAF1/CIP1) mRNA and downregulating the levels of mutant p53, cyclin D1 and CDK4 proteins and c-myc mRNA, and induce the differentiation of human hepatocacinoma cells.