Ulmus macrocarpa Hance Extracts Attenuated H₂O₂ and UVB-Induced Skin Photo-Aging by Activating Antioxidant Enzymes and Inhibiting MAPK Pathways

• Published in: International journal of molecular sciences Vol. 18; no. 6; p. 1200
• Main Authors: Choi, Sun-Il, Lee, Jin-Ha, Kim, Jae-Min, Jung, Tae-Dong, Cho, Bong-Yeon, Choi, Seung-Hyun, Lee, Dae-Won, Kim, Jinkyung, Kim, Jong-Yea, Lee, Ok-Hawn
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.06.2017; MDPI
• Subjects: Aging; Aging (artificial); Animals; Antioxidants; Antioxidants - pharmacology; Bioactive compounds; c-Jun protein; Catalase; Catalase - genetics; Catalase - metabolism; Cell Death - drug effects; Collagen; Cytotoxicity; Enzyme Activators - pharmacology; Enzymes; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Functional materials; Gene Expression Regulation, Enzymologic - drug effects; Glutathione peroxidase; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Glutathione reductase; Hairless; High performance liquid chromatography; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; JNK protein; Kinases; Male; Manganese; MAP kinase; MAP Kinase Signaling System - drug effects; Matrix metalloproteinase; Matrix metalloproteinases; Metabolic disorders; Mice; Mitochondria; Morphology; Oral administration; Oxidative stress; Peroxidase; Phosphorylation; Photodiodes; Plant Extracts - pharmacology; Protein kinase; Proteins; Reactive Oxygen Species - metabolism; Reductases; Senescence; Signal transduction; Skin; Skin - drug effects; Skin - metabolism; Skin - pathology; Skin - radiation effects; Skin Aging - drug effects; Skin Aging - radiation effects; Superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Transcription factors; Ulmus - chemistry; Ultraviolet radiation; Ultraviolet Rays - adverse effects; anti-aging; antioxidant; hairless mice; Ulmus macrocarpa Hance extracts; human dermal fibroblasts
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms18061200

To protect from reactive oxygen species (ROS) damages, skin cells have evolved to have antioxidant enzymes, such as copper and zinc-dependent superoxide dismutase (SOD1), mitochondrial manganese-dependent superoxide dismutase (SOD2), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GR), and suppressed the expression of matrix metalloproteinases (MMPs) through the mitogen-activated protein kinase (MAPK) signaling pathways, such as c-Jun N-terminal kinase (JNK) and p38. Bioactive compounds analyses were performed using a high-performance liquid chromatography-photodiode array detector (HPLC-PDA) system. The antioxidant activity of Hance (UMH) extracts was estimated in vitro. The anti-aging activity of UMH extracts was estimated in vivo using the SKH-1 hairless mice. The UMH extracts reduced the H₂O₂-induced intracellular ROS production and the cell damages in human dermal fibroblasts (HDFs). Moreover, the H₂O₂-induced phosphorylation of JNK and p38 was detected in HDF and UMH extracts blocked the phosphorylation. These results suggest that UMH extracts can reduce the expression of MMPs and the reduced MMPs lead to the inhibition of collagen degradation. In addition, oral administration of the UMH extracts decreased the depth, thickness, and length of wrinkles on UVB exposed hairless mice. Therefore, UMH extracts play an advantage of the functional materials in antioxidant and anti-aging of skin.