Immobilization of recombinant Escherichia coli cells expressing glucose isomerase using modified diatomite as a carrier for effective production of high fructose corn syrup in packed bed reactor

• Published in: Bioprocess and biosystems engineering Vol. 44; no. 8; pp. 1781 - 1792
• Main Authors: Jin, Li-Qun, Chen, Xian-Xiao, Jin, Yi-Ting, Shentu, Jun-Kang, Liu, Zhi-Qiang, Zheng, Yu-Guo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2021; Springer Nature B.V
• Subjects: Agricultural production; Aldose-Ketose Isomerases - chemistry; Bacterial Proteins; Bioreactors; Biotechnology; Chemistry; Chemistry and Materials Science; Cobalt - chemistry; Continuous production; Corn; Corn syrup; Crop yield; Diatomaceous earth; Diatomaceous Earth - chemistry; E coli; Environmental Engineering/Biotechnology; Enzymes, Immobilized; Escherichia coli - metabolism; Flow stability; Flow velocity; Food Science; Fructose; Fructose - chemistry; Glucose; Glucose isomerase; High Fructose Corn Syrup - chemistry; Hydrogen-Ion Concentration; Immobilization; Immobilized cells; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Ions; Magnesium - chemistry; Microscopy, Electron, Scanning; Packed beds; Reactors; Recombinant Proteins - chemistry; Research Paper; Syrup; Syrups; Temperature; Immobilization; Modified diatomite; Packed bed bioreactor; Glucose isomerase; High fructose corn syrup
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-021-02560-4

To improve the operational stability of glucose isomerase in E. coli TEGI-W139F/V186T, the immobilized cells were prepared with modified diatomite as a carrier and 74.1% activity of free cells was recovered after immobilization. Results showed that the immobilized cells still retained 86.2% of the initial transformational activity after intermittent reused 40 cycles and the yield of D-fructose reached above 42% yield at 60 °C. Moreover, the immobilized cells were employed in the continuous production of High Fructose Corn Syrup (HFCS) in a recirculating packed bed reactor for 603 h at a constant flow rate. It showed that the immobilized cells exhibited good operational stability and the yield of D-fructose retained above 42% within 603 h. The space–time yield of high fructose corn syrup reached 3.84 kg L −1  day −1 . The investigation provided an efficient immobilization method for recombinant cells expressing glucose isomerase with higher stability, and the immobilized cells are a promising biocatalyst for HFCS production.