Paraffin processing of stented arteries using a postfixation dissolution of metallic and polymeric stents

Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic rel...

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Published inCardiovascular pathology Vol. 25; no. 6; pp. 483 - 488
Main Authors Fishbein, Ilia, Welch, Tre, Guerrero, David T., Alferiev, Ivan S., Adamo, Richard F., Chorny, Michael, Gupte, Rohit K., Tang, Yanqing, Levy, Robert J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.2016
Subjects
Animals
Arterial tissue
Coronary Vessels - pathology
Histological processing
Immunohistochemistry
Male
Models, Animal
Paraffin embedding
Paraffin Embedding - methods
Polyesters - chemistry
Rats
Rats, Sprague-Dawley
Solubility
Staining and Labeling
Stainless Steel - chemistry
Stents
Tissue Fixation
Immunohistochemistry
Histological processing
Arterial tissue
Stents
Paraffin embedding
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Summary:Studying the morphology of the arterial response to endovascular stent implantation requires embedding the explanted stented artery in rigid materials such as poly(methyl methacrylate) to enable sectioning through both the in situ stent and the arterial wall, thus maintaining the proper anatomic relationships. This is a laborious, time-consuming process. Moreover, the technical quality of stained plastic sections is typically suboptimal and, in some cases, precludes immunohistochemical analysis. Here we describe a novel technique for dissolution of metallic and plastic stents that is compatible with subsequent embedding of “destented” arteries in paraffin, fine sectioning, major staining protocols, and immunohistochemistry.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1054-8807
1879-1336
DOI:10.1016/j.carpath.2016.08.002