Simultaneous extraction of antibiotic and estrogen from animal blood serum using aqueous two-phase systems as predictor of environmental impact

• Published in: Environmental science and pollution research international Vol. 27; no. 23; pp. 28536 - 28544
• Main Authors: Díaz-Quiroz, Carlos, Hernández-Chávez, Juan Francisco, Ulloa-Mercado, Gabriela, Deive, Francisco, Gortáres-Moroyoqui, Pablo, Molina-Barrios, Ramón Miguel
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer Nature B.V
• Subjects: 17β-Estradiol; Advances in Environmental Biotechnology and Engineering 2018; Antibiotics; Antimicrobial resistance; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Binary systems; Blood; Capillary electrophoresis; Chlortetracycline; Citric acid; Dosage; Earth and Environmental Science; Ecotoxicology; Electrophoresis; Environment; Environmental Chemistry; Environmental Health; Environmental impact; Environmental science; Estrogens; Hormones; Ionic liquids; Ions; Liquid-liquid equilibrium; Liquid-liquid extraction; Optimization; Phase diagrams; Recovery; Sex hormones; Solvents; Toxicity; Waste Water Technology; Water Management; Water Pollution Control; Hormone; Biphasic system; Antibiotic; Capillary electrophoresis; Extraction; Wastewater treatment; Aqueous two-phase system
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-020-07770-z

Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid and low-cost method for determination of hormone estradiol and antibiotic chlortetracycline in bovine and porcine blood serum by aqueous two-phase system (ATPS) extraction and capillary electrophoresis quantification. ATPS based on ionic liquid cholinium alaninate and citrate salt along with mixtures of protic and aprotic polar solvents were evaluated in terms of recovery of extraction (%R). The liquid-liquid equilibrium, phase diagrams, and tie lines are discussed. Antibiotic migrated to solvent-rich phase ( R  ≈ 89.0%) to all systems. Estradiol migrates to ionic liquid-rich phase; however, addition of 10% methanol changed partition to solvent-rich phase ( R  ≈ 89.7%). The method has high recovery and cleanliness, is cost-efficient, scalable, and hence is adequate for screening of antibiotics and hormones tested in animal blood serum for dosage optimization and to predict their environment.