Comparison of three immunoglobulin G assays for the diagnosis of failure of passive transfer of immunity in neonatal alpacas

• Published in: Journal of veterinary diagnostic investigation Vol. 25; no. 1; p. 91
• Main Authors: Pinn, Toby L, Gagliardo, Lucille F, Purdy, Steve R, Appleton, Judith A, Stokol, Tracy
• Format: Journal Article
• Language: English
• Published: United States 01.01.2013
• Subjects: Animals; Animals, Newborn - blood; Animals, Newborn - immunology; Camelids, New World - blood; Camelids, New World - immunology; Female; Immunity, Maternally-Acquired - immunology; Immunoassay - methods; Immunoassay - veterinary; Immunodiffusion - veterinary; Immunoglobulin G - blood; Immunoglobulin G - immunology; Male; Nephelometry and Turbidimetry - veterinary; Prospective Studies; Reproducibility of Results
• ISSN: 1943-4936
• DOI: 10.1177/1040638712472182

Measurement of serum immunoglobulin G (IgG) is used for the assessment of passive transfer of immunity in neonatal crias, with an IgG concentration <10 g/l being suggestive of failure of passive transfer (FPT). The purpose of the current study was to determine whether 3 commercially available immunologic assays yielded comparable results for IgG in alpacas. Serum samples from 91 alpacas were used and were stored frozen until batch analysis on the same day with the 3 assays. Immunoglobulin G was measured by radial immunodiffusion (RID) and 2 immunoturbidimetric (IT) assays (IT1, configured for automated chemistry analyzers; IT2, a point-of-care test). Median IgG concentrations were significantly different between the 3 assays, with the RID (median: 15 g/l) and IT1 (median: 16 g/l) assays, which used the same standard, yielding significantly higher IgG values than IT2 (median: 11 g/l). Results indicated a diagnostic discordance in 1-17% of samples at an IgG threshold of 10 g/l. Protein electrophoresis revealed that the RID and IT1 standard contained mostly albumin (>60%), whereas the IT2 standard consisted of beta and gamma globulins. The discrepant results between assays IT1 and IT2 were eliminated when the same standard was used (IT1: median 11 g/l; IT2: 10 g/l; n = 19 and 17, respectively). The IT1 assay had the highest precision, while the RID assay had the lowest. The results indicate that camelid IgG measurement is highly dependent on the assay standard and is not directly comparable between assays, potentially resulting in underdiagnosis of FPT in some crias.