QTL mapping of resistance in lentil (Lens culinaris ssp. culinaris) to ascochyta blight (Ascochyta lentis)

• Published in: Plant breeding Vol. 125; no. 5; pp. 506 - 512
• Main Authors: Taylor, P. W. J., Ades, P. K., Ford, R.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.10.2006; Blackwell
• Subjects: AFLP; Agronomy. Soil science and plant productions; Ascochyta; Ascochyta lentis; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Lens culinaris; linkage map; QTL; stem pigmentation; Varietal selection. Specialized plant breeding, plant breeding aims; stem pigmentation; Plant pathogen; Mycosis; QTL; Lens culinaris; Stem; Fungi; Dicotyledones; Linkage group; Angiospermae; Fungi Imperfecti; Thallophyta; Genetic mapping; Pigmentation; Ascochyta lentis; Fungus resistance; Infection; Quantitative trait loci; Grain legume; Lentil; Leguminosae; Vegetable crop; Genetic improvement; AFLP; Spermatophyta; Ascochyta; Amplified fragment length polymorphism marker; linkage map
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2006.01259.x

Quantitative trait locus (QTL) analysis of ascochyta blight resistance in lentil was conducted using genomic maps developed from two F2 populations, viz. ILL5588/ILL7537 and ILL7537/ILL6002. Five QTLs for ascochyta blight resistance were identified by composite interval mapping (CIM) across four linkage groups (LG) in population ILL5588/ILL7537. Three QTLs were identified by CIM in population ILL7537/ILL6002 (two in close proximity on LGI and one on LGII). Two of these coincided with regions identified using multiple interval mapping (MIM) and were shown to be conditioned by dominant and partial dominant gene action. Together, they accounted for approximately 50% of the phenotypic variance of disease severity. Comparison between the two populations revealed a potentially common QTL and several common regions that contained markers significantly associated with resistance. This study demonstrated the transferability of QTLs among populations and identified markers closely linked to the major QTL that may be useful for future marker‐assisted selection for disease resistance.