Diversity of bacteria in different life stages and their impact on the development and reproduction of Zeugodacus tau (Diptera: Tephritidae)

• Published in: Insect science Vol. 28; no. 2; pp. 363 - 376
• Main Authors: Noman, Md. Shibly, Shi, Ge, Liu, Li‐Jun, Li, Zhi‐Hong
• Format: Journal Article
• Language: English
• Published: Australia Wiley Subscription Services, Inc 01.04.2021
• Subjects: Animals; Antibiotics; Bacteria; Bacteria - classification; Bacteria - isolation & purification; bacterial diversity; Developmental stages; Eggs; Enterobacter; Environmental management; Female; fitness; Fruit flies; Gene sequencing; High-Throughput Nucleotide Sequencing; Insects; Klebsiella; Larva - growth & development; Larva - microbiology; Larvae; Male; Microbiota; Oviposition; Ovum - growth & development; Ovum - microbiology; Pupa - growth & development; Pupa - microbiology; Reproduction; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; rRNA 16S; Sequence analysis; symbiotic bacteria; Tephritidae - growth & development; Tephritidae - microbiology; Tephritidae - physiology; Zeugodacus tau; bacterial diversity; Zeugodacus tau; oviposition; antibiotics; fitness; symbiotic bacteria
• ISSN: 1672-9609; 1744-7917
• DOI: 10.1111/1744-7917.12768

Fruit flies usually harbor diverse communities of bacteria in their digestive systems, which are known to play a significant role in their fitness. However, little information is available on Zeugodacus tau, a polyphagous pest worldwide. This study reports the first extensive analysis of bacterial communities in different life stages and their effect on the development and reproduction of laboratory‐reared Z. tau. Cultured bacteria were identified using the conventional method, and all bacteria were identified by high‐throughput technologies (16S ribosomal RNA gene sequencing of V3‒V4 region). A total of six bacterial phyla were identified in larvae, pupae, and male and female adult flies, which were distributed into 14 classes, 32 orders, 58 families and 96 genera. Proteobacteria was the most represented phylum in all the stages except larvae. Enterobacter, Klebsiella, Providencia, and Pseudomonas were identified by conventional and next‐generation sequencing analysis in both male and female adult flies, and Enterobacter was found to be the main genus. After being fed with antibiotics from the first instar larvae, bacterial diversity changed markedly in the adult stage. Untreated flies laid eggs and needed 20 days before oviposition while the treated flies showed ovary development inhibited and were not able to lay eggs, probably due to the alteration of the microbiota. These findings provide the cornerstone for unexplored research on bacterial function in Z. tau, which will help to develop an environmentally friendly management technique for this kind of harmful insect.