DNA Detection through Signal Amplification by Using NADH:Flavin Oxidoreductase and Oligonucleotide-Flavin Conjugates as Cofactors

Cofactors on the case: DNA detection by using an enzyme and its cofactor attached to an oligonucleotide is described. If the enzyme is functional only when the cofactor–oligonucleotide conjugate is not hybridized, substrate conversion monitored by fluorescence spectroscopy can be used as a reaction...

Full description

Saved in:
Bibliographic Details
Published inAngewandte Chemie (International ed.) Vol. 44; no. 18; pp. 2764 - 2767
Main Authors Simon, Philippe, Dueymes, Cécile, Fontecave, Marc, Décout, Jean-Luc
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 29.04.2005
WILEY‐VCH Verlag
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:Cofactors on the case: DNA detection by using an enzyme and its cofactor attached to an oligonucleotide is described. If the enzyme is functional only when the cofactor–oligonucleotide conjugate is not hybridized, substrate conversion monitored by fluorescence spectroscopy can be used as a reaction probe for hybridization (see scheme). Furthermore, as the enzyme turns over many copies of the substrate, the signal is greatly amplified.
Bibliography:istex:F5B920E717C04A8C9ADA83B781E24CECFA560CDC
NADH=nicotinamide adenine dinucleotide, reduced form. This work was supported by the CNRS program "Puces à ADN".
ArticleID:ANIE200461145
ark:/67375/WNG-DGVSJ0Q1-D
NADH=nicotinamide adenine dinucleotide, reduced form. This work was supported by the CNRS program “Puces à ADN”.
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200461145