Analysis of circulating immune complexes isolated from plasma, cerebrospinal fluid and urine

The protein nature of soluble immune complexes (IC) from fresh plasma, cerebrospinal fluid (CSF), and urine was studied by combining several analytical and biochemical techniques. In plasma and CSF, free immunoglobulins G were separated from larger IC by gel filtration with a fast protein liquid chr...

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Bibliographic Details
Published inElectrophoresis Vol. 12; no. 7-8; p. 478
Main Authors Wiederkehr, F, Büeler, M R, Vonderschmitt, D J
Format Journal Article
LanguageEnglish
Published Germany 1991
Subjects
Antigen-Antibody Complex - analysis
Antigen-Antibody Complex - cerebrospinal fluid
Antigen-Antibody Complex - isolation & purification
Electrophoresis, Gel, Two-Dimensional - methods
Humans
Molecular Weight
Staining and Labeling
Urinalysis
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Summary:The protein nature of soluble immune complexes (IC) from fresh plasma, cerebrospinal fluid (CSF), and urine was studied by combining several analytical and biochemical techniques. In plasma and CSF, free immunoglobulins G were separated from larger IC by gel filtration with a fast protein liquid chromatographic system. In urine, IC were separated by precipitation with polyethylene glycol. IC were further purified by protein-A and protein-G affinity chromatography and analyzed by two-dimensional gel electrophoresis. Apart from plasma samples from healthy donors, IC from cases with macrocreatine kinase type 1 and multiple sclerosis were analyzed. For CSF two cases of multiple sclerosis and for urine one case with urinary tract infection are shown. The method can be used for the examination of IC of unknown protein composition in body fluids.
ISSN:0173-0835
DOI:10.1002/elps.1150120705