Enhanced Cadmium Accumulation in Transgenic Tobacco Expressing the Phytochelatin Synthase Gene of Cynodon dactylon L
Bermudagrass (Cynodon dactylon L. cv. Goldensun) is highly resistant to and accumulates large amounts of cadmium (Cd). A phytochelatin synthase (PCS) cDNA (CdPCS1) was isolated from this grass by rapid amplification of cDNA ends. The putative CdPCS1 protein shared a high homology with PCS from other...
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Published in | Journal of integrative plant biology Vol. 48; no. 8; pp. 928 - 937 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Melbourne, Australia
Blackwell Publishing Asia
01.08.2006
Graduate School of the Chinese Academy of Sciences, Beijing 100039, China%Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China |
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Online Access | Get full text |
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Summary: | Bermudagrass (Cynodon dactylon L. cv. Goldensun) is highly resistant to and accumulates large amounts of cadmium (Cd). A phytochelatin synthase (PCS) cDNA (CdPCS1) was isolated from this grass by rapid amplification of cDNA ends. The putative CdPCS1 protein shared a high homology with PCS from other plants, with 79% homology at the N‐terminal and 47% homology at the C‐terminal. However, 16 Cys residues were found at the C‐terminal of CdPCS1, and among these residues, three positions were different from other PCS proteins. Semiquantitative reverse transcription‐polymerase chain reaction analysis showed that Cd stress induced CdPCS1 expression in both roots and leaves in Bermudagrass. We verified that CdPCS1 plays an important role in Cd tolerance in yeast cells by expressing the gene in ABDE1, a Cd‐sensitive mutant. CdPCS1 was then introduced into tobacco plants. The phytochelatin level in some transgenic tobacco lines increased 3.88‐fold more than in wild type plants and Cd accumulation in these transgenic plants was enhanced 3.21‐fold accordingly. The results suggested that CdPCS1 could be used as a gene element for phytoremediation in the future.
(Managing editor: Ya‐Qin Han) |
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Bibliography: | ark:/67375/WNG-0KSZ9WMS-D ArticleID:JIPB314 istex:833DE9EE477C1BEEA78122DF291ECD238D9B7AE4 Supported by the Special Project of Transgenic Plants Research (JY03A2001). ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1672-9072 1744-7909 |
DOI: | 10.1111/j.1744-7909.2006.00314.x |