Combination of resistance to Verticillium longisporum from zero erucic acid Brassica oleracea and oilseed Brassica rapa genotypes in resynthesized rapeseed (Brassica napus) lines

• Published in: Plant breeding Vol. 126; no. 6; pp. 596 - 602
• Main Authors: Rygulla, W., Friedt, W., Seyis, F., Lühs, W., Eynck, C., Von Tiedemann, A., Snowdon, R. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2007; Blackwell
• Subjects: Agronomy. Soil science and plant productions; B. oleracea; B. rapa; Biological and medical sciences; Brassica; Brassica napus; Brassica oleracea; Brassica rapa; erucic acid; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics and breeding of economic plants; Plant material; resistance; resynthesized rapeseed; Varietal selection. Specialized plant breeding, plant breeding aims; Verticillium longisporum; Brassica rapa; Oilseed; Plant pathogen; Brassica napus var. oleifera; B. rapa; Monounsaturated fatty acid; Erucic acid; Fungi; Cruciferae; Dicotyledones; Angiospermae; Fungi Imperfecti; Genotype; resynthesized rapeseed; Fungus resistance; resistance; Verticillium longisporum; Brassica oleracea; Vegetable crop; Rapeseed; Genetic improvement; Brassica napus; Spermatophyta; B. oleracea; Oil plant (vegetal)
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2007.01414.x

Resynthesized (RS) forms of rapeseed (Brassica napus L.; genome AACC, 2n = 38) generated from interspecific hybridization between suitable genotypes of its diploid progenitors Brassica rapa L. (syn. campestris; genome AA, 2n = 20) and Brassica oleracea L. (CC, 2n = 18) represent a potentially useful resource to introduce resistance against the fungal pathogen Verticillium longisporum into the gene pool of oilseed rape. Numerous cabbage (B. oleracea) accessions are known with resistance to V. longisporum; however, B. oleracea generally has high levels of erucic acid and glucosinolates in the seed, which reduces the suitability of resulting RS rapeseed lines for oilseed rape breeding. In this study resistance against V. longisporum was identified in the cabbage accession Kashirka 202 (B. oleracea convar. capitata), a zero erucic acid mutant, and RS rapeseed lines were generated by crossing the resistant genotype with two spring turnip rape accessions (B. rapa ssp. olerifera) with zero erucic acid. One of the resulting zero erucic acid RS rapeseed lines was found to have a high level of resistance to V. longisporum compared with both parental accessions and with B. napus controls. A number of other zero erucic acid RS lines showed resistance levels comparable to the parental accessions. In the most resistant RS lines the resistance and zero erucic acid traits were combined with variable seed glucosinolate contents. Erucic acid‐free RS rapeseed with moderate seed glucosinolate content represents an ideal basic material for introgression of quantitative V. longisporum resistance derived from B. oleracea and B. rapa into elite oilseed rape breeding lines.