Reduction in Listeria monocytogenes and spoilage bacteria on smoked catfish using X‐ray treatments

• Published in: Letters in applied microbiology Vol. 54; no. 6; pp. 524 - 529
• Main Authors: Mahmoud, B.S.M, Coker, R, Su, Y.‐C
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2012; Blackwell
• Subjects: Animals; bacteria; Biological and medical sciences; catfish; Catfishes - microbiology; Cold Temperature; Colony Count, Microbial; Cooking - methods; fillets; Food Handling - methods; Food Irradiation; Food Storage; Fundamental and applied biological sciences. Psychology; inactivation; Listeria monocytogenes; Listeria monocytogenes - growth & development; Listeria monocytogenes - radiation effects; Microbiology; refrigeration; Seafood - microbiology; shelf life; smoked catfish; spoilage; temperature; X-ray radiation; X-Rays; Reduction; Treatment; Applied microbiology; Radiation; smoked catfish; Listeria monocytogenes; Bacteria; X ray; X-ray radiation; Inactivation; Biological contamination
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/j.1472-765X.2012.03242.x

Aims: To determine the efficacy of X‐ray processes in inactivating L. monocytogenes levels in smoked catfish during storage at 5°C and to determine the effects of X‐ray doses on controlling the growth of spoilage bacteria on smoked catfish during storage at 5°C for up to 5 weeks. Methods and Results: Smoked catfish fillets inoculated with L. monocytogenes were treated with 0·0–2·0 kGy X‐ray and stored at 5°C for 5 weeks. The negative controls (uninoculated/untreated) and uninoculated samples treated with the lowest (0·1 kGy) and highest (2·0 kGy) doses were stored at 5°C and tested for psychrotrophs count during the 5 weeks of storage. The initial L. monocytogenes population on smoked catfish was significantly (P < 0·05) reduced to undetectable level by a treatment of 1·0 kGy or higher. The initial psychrotrophs count on smoked catfish was significantly reduced from 4·7 CFU g−1 to below the detectable level by a treatment with 2·0 kGy. Conclusions: Smoked catfish treated with 2·0 kGy X‐ray had no detectable L. monocytogenes throughout 35 days of storage at 5°C. A treatment with 2·0 kGy X‐ray also kept the levels of psychrotrophs in the smoked catfish within the acceptable level until 35 days. Significance and Impact of the Study: The results of this investigation indicate that X‐ray at 2·0 kGy can eliminate L. monocytogenes and extend the shelf life of smoked catfish stored at refrigeration temperature.