A technique for detecting antifungal activity of proteins separated by polyacrylamide gel electrophoresis

A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the poly...

Full description

Saved in:
Bibliographic Details
Published inElectrophoresis Vol. 12; no. 6; p. 442
Main Authors De Bolle, M F, Goderis, I J, Terras, F R, Cammue, B P, Broekaert, W F
Format Journal Article
LanguageEnglish
Published Germany 1991
Subjects
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Antimicrobial Cationic Peptides
Chitinases - chemistry
Chitinases - pharmacology
Electrophoresis, Polyacrylamide Gel
Lectins - chemistry
Lectins - pharmacology
Nicotiana - enzymology
Plant Lectins
Plant Proteins - chemistry
Plant Proteins - pharmacology
Plants, Toxic
Online AccessGet more information

Cover

More Information
Summary:A technique was developed for the detection of antifungal activity of proteins after discontinuous polyacrylamide gel electrophoresis under native conditions. The antifungal activity is detected as growth inhibition zones in a homogeneous fungal lawn, grown in an agar layer spread on top of the polyacrylamide gel. The position of proteins with antifungal activity can be determined on a diffusion blot prepared from the same gel. The technique is illustrated for three antifungal plant proteins, i.e. alpha-purothionin, Urtica dioica agglutinin, and tobacco chitinase.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150120612