Low Inflammatory Activation by Self-Assembling Rosette Nanotubes in Human Calu-3 Pulmonary Epithelial Cells
Rosette nanotubes (RNT) are a new class of metal‐free organic nanotubes synthesized through self‐assembly. Because of the wide range of potential biomedical applications associated with these materials, it is necessary to evaluate their potential in vitro toxicity. Here the cytotoxicity of a lysine‐...
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Published in | Small (Weinheim an der Bergstrasse, Germany) Vol. 4; no. 6; pp. 817 - 823 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.06.2008
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
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Summary: | Rosette nanotubes (RNT) are a new class of metal‐free organic nanotubes synthesized through self‐assembly. Because of the wide range of potential biomedical applications associated with these materials, it is necessary to evaluate their potential in vitro toxicity. Here the cytotoxicity of a lysine‐functionalized nanotube (RNT‐K) in a human Calu‐3 pulmonary epithelial cell line is investigated. The cells were treated with media only (control), lysine (50 mg mL−1), RNT‐K (1, 5, and 50 µg mL−1), Min‐U‐Sil quartz microparticles (QM; 80 µg mL−1), and lipopolysaccharide (LPS; 1 µg mL−1). The supernatants were analyzed at 1, 6, and 24 h after treatment for the expression of three proinflammatory mediators: IL‐8, TNF‐α and EMAP‐II. Cellular viability determined with the Trypan blue assay is significantly reduced in the QM and high‐dose RNT‐treated groups. TNF‐α and EMAP‐II are undetectable by enzyme‐linked‐immunosorbent assay (ELISA) in the supernatant of all groups. Although IL‐8 concentrations do not differ between treatments, its concentrations increase with time within each of the groups. Quantitative reverse‐transcriptase polymerase chain reaction (qRTPCR) of IL‐8 mRNA shows increased expression in the high‐dose RNT‐treated groups at both 1 and 6 h, while an adhesion molecule, ICAM‐1 mRNA, shows the greatest increase at 6 h in the QM‐treated group. In summary, RNT‐K neither reduces cell viability at moderate doses nor does it induce a time‐dependent inflammatory response in pulmonary epithelial cells in vitro.
Toxicity of organic nanotubes: Rosette nanotubes (see C and D in figure) self‐assemble from structures shown in A and B and may have a range of biomedical applications. It is found that the rosette nanotubes have low toxicity in human Calu‐3 pulmonary epithelial cells. These experiments add to the burgeoning field of toxicology on the nanoscale. |
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Bibliography: | istex:2CB6985E61399D16D3C0DA77F5C1D8E3C3D41473 ArticleID:SMLL200700700 ark:/67375/WNG-SK2L1ZQ9-3 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 1613-6810 1613-6829 |
DOI: | 10.1002/smll.200700700 |