Black tea extract suppresses transformation of aryl hydrocarbon receptor induced by dioxin
Dioxins cause various adverse effects through binding to an aryl hydrocarbon receptor (AhR) and transformation of the receptor. In this study, we investigated whether black tea extract suppresses AhR transformation. Dried black tea leaves were extracted with 75% ethanol, and the extract was pretreat...
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Published in | BioFactors (Oxford) Vol. 21; no. 1-4; pp. 367 - 369 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
IOS Press
2004
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Subjects | |
Online Access | Get full text |
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Summary: | Dioxins cause various adverse effects through binding to an aryl hydrocarbon receptor (AhR) and transformation of the receptor. In this study, we investigated whether black tea extract suppresses AhR transformation. Dried black tea leaves were extracted with 75% ethanol, and the extract was pretreated to the rat liver cytosol fraction 10 min prior to addition of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD). Transformed AhR was detected by electrophoretic gel mobility shift assay. Black tea extract suppressed AhR transformation in a dose‐dependent manner, and the IC50 value against 1 nM TCDD‐induced AhR transformation was 8.9 μ/ml. The result suggests that intake of black tea has a potential to suppress the AhR transformation, leading protection from dioxin toxicity. |
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Bibliography: | ArticleID:BIOF552210170 istex:850366AC6ED5742687C90E3463F11ACBC608F173 Japan Society for the Promotion of Science for Young Scientists (I. F.). ark:/67375/WNG-510PSHPH-N ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Conference-1 ObjectType-Feature-3 content type line 23 SourceType-Conference Papers & Proceedings-2 |
ISSN: | 0951-6433 1872-8081 |
DOI: | 10.1002/biof.552210170 |