Genomic behavior of hybrid combinations between elephant grass and pearl millet

The objective of this work was to evaluate the genomic behavior of hybrid combinations between elephant grass (Pennisetum purpureum) and pearl millet (P. glaucum). Tetraploid (AAA'B) and pentaploid (AA'A'BB) chromosome races resulting from the backcross of the hexaploid hybrid to its...

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Published inPesquisa agropecuaria brasileira Vol. 46; no. 7; pp. 712 - 719
Main Authors Leão, Fernando Ferreira, Davide, Lisete Chamma, Campos, José Marcello Salabert de, Pereira, Antonio Vander, Bustamante, Fernanda de Oliveira
Format Journal Article
LanguageEnglish
Published Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira 01.07.2011
Embrapa Informação Tecnológica
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Summary:The objective of this work was to evaluate the genomic behavior of hybrid combinations between elephant grass (Pennisetum purpureum) and pearl millet (P. glaucum). Tetraploid (AAA'B) and pentaploid (AA'A'BB) chromosome races resulting from the backcross of the hexaploid hybrid to its parents elephant grass (A'A'BB) and pearl millet (AA) were analyzed as to chromosome number and DNA content. Genotypes of elephant grass, millet, and triploid and hexaploid induced hybrids were compared. Pentaploid and tetraploid genomic combinations showed high level of mixoploidy, in discordance with the expected somatic chromosome set. The pentaploid chromosome number ranged from 20 to 34, and the tetraploid chromosome number from 16 to 28. Chromosome number variation was higher in pentaploid genomic combinations than in tetraploid, and mixoploidy was observed among hexaploids. Genomic combinations 4x and 5x are mixoploid, and the variation of chromosome number within chromosomal race 5x is greater than in 4x. O objetivo deste trabalho foi avaliar o comportamento genômico de combinações híbridas resultantes do cruzamento entre capim-elefante (Pennisetum purpureum) e milheto (P. glaucum). Raças cromossômicas tetraploides (AAA'B) e pentaploides (AA'A'BB), resultantes do retrocruzamento do híbrido hexaploide com seus parentais capim-elefante (A'A'BB) e milheto (AA), foram avaliadas quanto ao número cromossômico e ao conteúdo de DNA. Foram comparados os genótipos de capim-elefante, milheto e de híbridos triploides e hexaploides induzidos. As combinações genômicas pentaploides e tetraploides mostraram elevado grau de mixoploidia, em desacordo com o complemento cromossômico somático esperado. O número cromossômico dos pentaploides variou de 20 a 34, e o dos tetraploides de 16 a 28. A variação do número cromossômico foi maior nas combinações genômicas pentaploides do que nas tetraploides, e a mixoploidia foi verificada entre hexaploides. As combinações genômicas 4x e 5x são mixoploides, e a variação do número de cromossomos na raça cromossômica 5x é maior do que na 4x.
ISSN:0100-204X
1678-3921
0100-204X
DOI:10.1590/S0100-204X2011000700006