Pulmonary passage of canine adipose tissue-derived mesenchymal stem cells through intravenous transplantation in mouse model

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 25; no. 3; pp. e36 - 0
• Main Authors: Kwon, Jaeyeon, Kim, Mu-Young, Lee, Soojung, Lee, Jeongik, Yoon, Hun-Young
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Veterinary Science 01.05.2024; 대한수의학회
• Subjects: Adipose Tissue - cytology; Animals; Dogs; Male; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stem Cell Transplantation - veterinary; Mesenchymal Stem Cells - physiology; Mice; Mice, Inbred BALB C; Pulmonary Embolism - therapy; Pulmonary Embolism - veterinary; Research Report; 수의학; suspension volume; injection frequency; pulmonary thromboembolism; Intravenous injection; cell dose
• ISSN: 1229-845X; 1976-555X; 1976-555X
• DOI: 10.4142/jvs.23300

The intravenous administration of adipose tissue-derived mesenchymal stem cells (AdMSCs) in veterinary medicine is an attractive treatment option. On the other hand, it can result in severe complications, including pulmonary thromboembolism (PTE). The present study assessed the occurrence of PTE after the intravenous infusion of canine AdMSCs (cAdMSCs) into experimental animals. Five-week-old male BALB/c hairless mice were categorized into groups labeled A to G. In the control group (A), fluorescently stained 2 × 10 cAdMSCs were diluted in 200 μL of suspension and injected into the tail vein as a single bolus. The remaining groups included the following: group B with 5 × 10 cells, group C with 3 × 10 cells, group D with 1 × 10 cells, group E with 1 × 10 cells injected twice with a one-day interval, group F with 2 × 10 cells in 100 μL of suspension, and group G with 2 × 10 cells in 300 μL of suspension. Group D achieved a 100% survival rate, while none of the subjects in groups B and C survived ( = 0.002). Blood tests revealed a tendency for the D-dimer levels to increase as the cell dose increased ( = 0.006). The platelet count was higher in the low cell concentration groups and lower in the high cell concentration groups ( = 0.028). A histological examination revealed PTE in most deceased subjects (96.30%). PTE was verified, and various variables were identified as potential contributing factors, including the cell dose, injection frequency, and suspension volume.