Effectiveness of carbonic anhydrase inhibitor loaded nanoparticles in the treatment of diabetic retinopathy

• Published in: Biomedical physics & engineering express Vol. 10; no. 1; pp. 15002 - 15018
• Main Authors: Uğurlu, Nagihan, Erdal, Ebru, Malekghasemi, Soheil, Demirbilek, Murat
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.01.2024
• Subjects: acetazolamide; Adult; Aged; carbonic anhydrase inhibitor; Carbonic Anhydrase Inhibitors; Diabetes Mellitus; diabetic retinopathy; Diabetic Retinopathy - drug therapy; Diabetic Retinopathy - etiology; Diabetic Retinopathy - metabolism; Humans; Middle Aged; nanoparticles; Nanoparticles - chemistry; poly(3-hydroxybutyrate-co-3-hydroxyvalerate); Polyesters; Vascular Endothelial Growth Factor A; Young Adult; nanoparticles; diabetic retinopathy; carbonic anhydrase inhibitor; acetazolamide; poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
• ISSN: 2057-1976; 2057-1976
• DOI: 10.1088/2057-1976/acba9d

Diabetic Retinopathy (DRP) is a disease consisting of all the structural and functional changes that develop in the retinal layer of the eye due to diabetes. DRP is the most important cause of blindness between the ages of 20-74 in the world, and the most successful standard treatment option in the treatment of DRP is intravitreal injections. To synthesize acetazolamide loaded nanoparticles to be applied intravitreal treatment of DRP and to examine the efficacy of the nanoparticles. ACZ loaded PHBV nanoparticles (PHBV-ACZ NPs) formulations were prepared. Nanoparticles with a particle size of 253.20 ± 0.55 nm. A DRP model was established and characterized in HRMEC cells. The effect of the nanoparticles on permeability has been investigated and carrier proteins in BRB due to the development of DRP has been investigated. To establish the DRP model, HRMEC was stimulated with Recombinant human 165 Vascular Endothelial Growth Factor (VEGF), thereby temporarily reducing the expression levels of endothelial junction proteins, increasing the number of intercellular spaces in the monolayers of HRMECs. It was determined that after the cells were exposed to Carbonic anhydrase inhibitors (CAI) loaded nanoparticles, permeability decreased and protein expression increased.