A novel pseudovirus containing lewis X oligosaccharides with pD-L1 targeting to DCs induces T cells tolerance

The purpose of this study was to assess the potential of a novel pseudovirus targeting to dendritic cells (DCs) inducing T cells tolerance. To prove it, allogeneic DCs transfected of the novel pseudovirus containing Lewis X oligosaccharides with programmed death-ligand 1 were co-cultured with T cell...

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Bibliographic Details
Published inTransplantation Vol. 87; no. 9; p. 1305
Main Authors Wang, Xingsheng, Yang, Xuemei, Cui, Shehuai, Yan, Yiqun, Yang, Zhao
Format Journal Article
LanguageEnglish
Published United States 15.05.2009
Subjects
Coculture Techniques
Dendritic Cells - cytology
Dendritic Cells - immunology
Humans
Immune Tolerance
Lymphocyte Activation
Oligosaccharides - immunology
T-Lymphocytes - cytology
T-Lymphocytes - immunology
Transfection
Viruses - genetics
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Summary:The purpose of this study was to assess the potential of a novel pseudovirus targeting to dendritic cells (DCs) inducing T cells tolerance. To prove it, allogeneic DCs transfected of the novel pseudovirus containing Lewis X oligosaccharides with programmed death-ligand 1 were co-cultured with T cells. After mixed leukocyte reactions were performed, the proliferation, cytokines secretion, and activation marker expression of T cells were analyzed. The results demonstrated that the proliferation, cytokines secretion, and activation marker expression of T cells were suppressed after co-cultured with the pseudovirus transfected DCs. These findings suggested that DCs transfected with pseudovirus could induce T-cells tolerance, which provided a promising therapeutic means to negatively manipulate immune response in organ transplantation.
ISSN:1534-6080
DOI:10.1097/TP.0b013e3181a199ea