RNA sequestration to pathological lesions of neurodegenerative diseases

• Published in: Acta neuropathologica Vol. 96; no. 5; pp. 487 - 494
• Main Authors: GINSBERG, S. D, GALVIN, J. E, CHIU, T.-S, LEE, V. M.-Y, MASLIAH, E, TROJANOWSKI, J. Q
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.11.1998; Springer Nature B.V
• Subjects: Acridine orange; Acridine Orange - pharmacokinetics; Aged; Aged, 80 and over; Alzheimer's disease; Amyotrophic lateral sclerosis; Atrophy; Autopsy; Basal ganglia; Biological and medical sciences; Central nervous system diseases; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Dementia disorders; Female; Humans; Immunohistochemistry; Inclusion bodies; Lesions; Lewy bodies; Lewy body disease; Localization; Male; Medical sciences; Middle Aged; Movement disorders; Nerve Degeneration - metabolism; Nerve Degeneration - pathology; Nervous System Diseases - metabolism; Nervous System Diseases - pathology; Neurodegeneration; Neurodegenerative diseases; Neurofibrillary tangles; Neurofibrillary Tangles - metabolism; Neurofibrillary Tangles - pathology; Neurology; Paraffin; Paralysis; Parkinson's disease; Plaque, Amyloid - metabolism; Plaque, Amyloid - pathology; Ribonucleic acid; RNA; RNA - metabolism; Senile plaques; Species; Tissue Distribution; Human; Immunohistochemistry; Nervous system diseases; Senile plate; RNA; Alzheimer disease; Exploration; Confocal microscopy; Pick disease; Neurofibrillary tangle; Cerebral disorder; Pathology; Central nervous system disease; Laser; Degenerative disease; Cytoplasm
• ISSN: 0001-6322; 1432-0533
• DOI: 10.1007/s004010050923

Cytoplasmic RNA species have been identified recently within neurofibrillary tangles and senile plaques of Alzheimer's disease brain. To determine whether RNA sequestration is a common feature of other lesions found in progressive neurodegenerative disorders, acridine orange histofluorescence was employed, alone or in combination with immunohistochemistry and thioflavine-S staining to identify RNA species in paraffin-embedded brain tissue sections. Postmortem samples came from 39 subjects with the following diagnoses: Alzheimer's disease, amyotrophic lateral sclerosis/parkinsonism-dementia complex of Guam, corticobasal degeneration, diffuse Lewy body disease, normal controls, multiple system atrophy, Parkinson's disease, Pick's disease, progressive supranuclear palsy, and Shy-Drager syndrome. RNAs were detected in neurofibrillary tangles and neuritic senile plaques as well as in Pick bodies. However, Lewy bodies, Hirano bodies, and cytoplasmic glial inclusions did not contain abundant cytoplasmic RNA species. These observations demonstrate the selective localization of RNA species to distinct pathological lesions of neurodegenerative disease brains.