Distinct time effects of vaccination on long-term proliferative and IFN-gamma-producing T cell memory to smallpox in humans
Residual immunity to the smallpox virus raises key questions about the persistence of long-term immune memory in the absence of antigen, since vaccination ended in 1980. IFN-gamma-producing effector-memory and proliferative memory T cells were compared in 79 vaccinees 13-25 yr after their last immun...
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Published in | The Journal of experimental medicine Vol. 199; no. 11; pp. 1585 - 1593 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
The Rockefeller University Press
07.06.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Residual immunity to the smallpox virus raises key questions about the persistence of long-term immune memory in the absence of antigen, since vaccination ended in 1980. IFN-gamma-producing effector-memory and proliferative memory T cells were compared in 79 vaccinees 13-25 yr after their last immunization and in unvaccinated individuals. Only 20% of the vaccinees displayed both immediate IFN-gamma-producing effector-memory responses and proliferative memory responses at 6 d; 52.5% showed only proliferative responses; and 27.5% had no detectable vaccinia-specific responses at all. Both responses were mediated by CD4 and CD8 T cells. The vaccinia-specific IFN-gamma-producing cells were composed mainly of CD4Pos CD45RANeg CD11aHi CD27Pos and CCR7Neg T cells. Their frequency was low but could be expanded in vitro within 7 d. Time since first immunization affected their persistence: they vanished 45 yr after priming, but proliferative responses remained detectable. The number of recalls did not affect the persistence of residual effector-memory T cells. Programmed revaccination boosted both IFN-gamma and proliferative responses within 2 mo of recall, even in vaccinees with previously undetectable residual effector-memory cells. Such long-term maintenance of vaccinia-specific immune memory in the absence of smallpox virus modifies our understanding of the mechanism of persistence of long-term memory to poxviruses and challenges vaccination strategies. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Correspondence should be addressed to Brigitte Autran, Laboratoire d'Immunologie Cellulaire, Hôpital Pitié-Salpétrière, 83 Bd de l'Hôpital, 75013 Paris, France. Phone: 33-1-42-177481; Fax: 33-1-42-177490; email: brigitte.autran@psl.ap-hop-paris.fr B. Combadiere and A. Boissonnas contributed equally to this work. Abbreviations used in this paper: BCG, Bacille de Calmette et Guérin; BrdU, bromodeoxyuridine; SFC, spot-forming cell. |
ISSN: | 0022-1007 1540-9538 |
DOI: | 10.1084/jem.20032083 |